Shoot generation and callus induction of Dioscorea hispida Dennst by different plant growth hormones and basal media

Nor Hasima Mahmod, . and Zakiah Mustapha, . and Ahmad Hilman Ariffin Rusni, . and Nurul Anisah Ishak, . and Hafsah Jaafar, . Shoot generation and callus induction of Dioscorea hispida Dennst by different plant growth hormones and basal media. pp. 30-38. ISSN 2180-1983

Dioscorea hispida Dennst produces tuber which possess valuable medicinal properties but unsustainable harvesting has led to its reduction. The plant propagates slowly because of its low tuber sprouting rate. In average Dioscorea hispida Dennst tubers took approximately 60 d to break dormancy and sprout. Hence callus culture is proposed as a possible efficient type of culture for manipulation of this species. In the present study calli were induced from stem segments to evaluate callus culture potential of Dioscorea hispida Dennst. Results indicate that the combination of 1 mgL-naphthaleneacetic acid (NAA) 1 mgL- 6-benzylaminopurine (BAP) and 0.5 mgL- 2 4-dichlorophenoxyacetic acid (2 4-D) in Gamborg (B5) medium improved callus multiplication and differentiation in the stem culture as opposed to those in Murashige and Skoog (MS) medium. The findings from the present study provide the basis of callus culture protocol for stem explant of Dioscorea hispida Dennst with B5 being the more effective basal medium.

Full text available from: Official URL: https://journal.unisza.edu.my/agrobiotechnology/in...

Abstract

Dioscorea hispida Dennst produces tuber which possess valuable medicinal properties but unsustainable harvesting has led to its reduction. The plant propagates slowly because of its low tuber sprouting rate. In average Dioscorea hispida Dennst tubers took approximately 60 d to break dormancy and sprout. Hence callus culture is proposed as a possible efficient type of culture for manipulation of this species. In the present study calli were induced from stem segments to evaluate callus culture potential of Dioscorea hispida Dennst. Results indicate that the combination of 1 mgL-naphthaleneacetic acid (NAA) 1 mgL- 6-benzylaminopurine (BAP) and 0.5 mgL- 2 4-dichlorophenoxyacetic acid (2 4-D) in Gamborg (B5) medium improved callus multiplication and differentiation in the stem culture as opposed to those in Murashige and Skoog (MS) medium. The findings from the present study provide the basis of callus culture protocol for stem explant of Dioscorea hispida Dennst with B5 being the more effective basal medium.

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Item Type:	Article
AGROVOC Term:	Dioscorea hispida
AGROVOC Term:	Dioscoreaceae
AGROVOC Term:	Plant growth hormones
AGROVOC Term:	Callus
AGROVOC Term:	Callus culture
AGROVOC Term:	Sterilization
AGROVOC Term:	In vitro culture
AGROVOC Term:	Auxins
AGROVOC Term:	Cytokinins
AGROVOC Term:	Shoots
Depositing User:	Mr. AFANDI ABDUL MALEK
Last Modified:	24 Apr 2025 00:55
URI:	http://webagris.upm.edu.my/id/eprint/9424

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