-glucosidase inhibition DPPH scavenging activities and chemical analysis of polysaccharide extracts from Aquilaria sp. leaves

Nur Nasyitah Mohd Nasir, . and Zalilawati Mat Rashid, . and Wan Nadilah Wan Ahmad, . and Nor Hasima Mahmod, . -glucosidase inhibition DPPH scavenging activities and chemical analysis of polysaccharide extracts from Aquilaria sp. leaves. pp. 59-69. ISSN 2180-1983

Aquilaria sp. belongs to Thymelaeaceae family. This genus produces a unique fragrant wood locally known as gaharu. It is well known for usages in aromatherapy while very few scientific evidence on its usage as herbal medicine have been published. The current study was therefore conducted to determine the chemical characteristics as well as antioxidant and -glucosidase inhibition potential of carbohydrate-rich extracts (aqueous and polysaccharide extracts) from three Aquilaria species leaves (A. malaccensis A. sinensis and A. subintegra). The isolation of polysaccharide (PS) from the aqueous extract (AE) was conducted by ethanol precipitation prior to ultrasonic-assisted extraction. The total phenolic content (TPC) proximate and Fourier Transform Infrared Spectroscopy (FTIR) analysis were carried out to investigate the chemical characteristics while the bioactivities determined were 1 1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging and -glucosidase inhibition. As a result AE of A. malaccensisleaves showed the strongest -glucosidase (IC50: 0.038mg/mL) and DPPH (IC50: 0.084 mg/mL) inhibition while contained more TPC (61.16 mg GAE/g) compared to other two species. Furthermore PS of A. malaccensisleaves displayed even stronger -glucosidase inhibition (IC50: 0.014 mg/mL) compared to its respective AE and other PS.In conclusion this study primarily revealed the scientific evidence on the bioactive potential of PS of Aquilaria sp.

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Abstract

Aquilaria sp. belongs to Thymelaeaceae family. This genus produces a unique fragrant wood locally known as gaharu. It is well known for usages in aromatherapy while very few scientific evidence on its usage as herbal medicine have been published. The current study was therefore conducted to determine the chemical characteristics as well as antioxidant and -glucosidase inhibition potential of carbohydrate-rich extracts (aqueous and polysaccharide extracts) from three Aquilaria species leaves (A. malaccensis A. sinensis and A. subintegra). The isolation of polysaccharide (PS) from the aqueous extract (AE) was conducted by ethanol precipitation prior to ultrasonic-assisted extraction. The total phenolic content (TPC) proximate and Fourier Transform Infrared Spectroscopy (FTIR) analysis were carried out to investigate the chemical characteristics while the bioactivities determined were 1 1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging and -glucosidase inhibition. As a result AE of A. malaccensisleaves showed the strongest -glucosidase (IC50: 0.038mg/mL) and DPPH (IC50: 0.084 mg/mL) inhibition while contained more TPC (61.16 mg GAE/g) compared to other two species. Furthermore PS of A. malaccensisleaves displayed even stronger -glucosidase inhibition (IC50: 0.014 mg/mL) compared to its respective AE and other PS.In conclusion this study primarily revealed the scientific evidence on the bioactive potential of PS of Aquilaria sp.

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Item Type:	Article
AGROVOC Term:	Thymelaeaceae
AGROVOC Term:	Alpha glucosidase
AGROVOC Term:	Enzyme inhibitors
AGROVOC Term:	Antioxidants
AGROVOC Term:	Leaves
AGROVOC Term:	Extraction
AGROVOC Term:	Proximate analysis
AGROVOC Term:	Chemical analysis (methods)
AGROVOC Term:	Polysaccharides
AGROVOC Term:	Proximate composition
Depositing User:	Mr. AFANDI ABDUL MALEK
Last Modified:	24 Apr 2025 00:55
URI:	http://webagris.upm.edu.my/id/eprint/9427

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