Comparison on effects of temperature on different strains of phytase producing bacteria isolated from Malaysias Hot Spring

Tajul Afif Abdullah, . and Nurul Asma Hasliza Zulkifly, . and Nadiawati Alias, . and Anis Adilah Mustafa, . and Afnani Alwi@Ali, . Comparison on effects of temperature on different strains of phytase producing bacteria isolated from Malaysias Hot Spring. pp. 56-68. ISSN 2180-1983

The main purpose of this research was to find the best growth curve for bacterial growth and the optimum temperature for the production of phytase from different potential phytase producing bacterial strains. A total of four strains used were originally isolated from hot springs in Malaysia which were in Labis Johor (L3) Dusun Tua Selangor (RT) Ulu Legong Kedah (A) and Ranau Sabah (B9). Nutrient Agar (NA) and modified Phytase Screening Medium (PSM) liquid media were used for the culture enrichment while optimisation was carried out through batch culture method using a shake-flask scale. Strains growth and enzyme activity were quantitatively measured at different temperatures at (30C and 37C) values. Enzyme activity was determined according to the reaction of the phytase with its substrate (sodium phytate) and expressed in units of phytase activity (U/ mL). As for the overall strain L3 (from Labis Johor) exhibit promising rate of Pi released in the media at 30Cand 37C with optimum phytase activity values of 0.2047 U/mL and 0.2195 U/mL respectively. The pH of the cultures was also measured where it shows that strains grown in cultures at 37C produced a higher phytase activity and resulting a lower reading of pH compared when grown at 30C. All around L3 strains has the lowest value of pH when cultured at 30C and 37C with the pH value of 3.62 and 2.37 respectively. From the result obtained the lower pH indicates the process of phytic acid degradation take place by the phytase in producing in organic phosphate (Pi) due to the accumulation of organic acid. Since these bacterial strains were originally taken from Hot Springs further analysis of temperature optimization using 55C and even 60C should be carried out. In the future biochemical research and molecular identification may also be carried out to identify molecular identity in the strains.

Full text available from: Official URL: https://journal.unisza.edu.my/agrobiotechnology/in...

Abstract

The main purpose of this research was to find the best growth curve for bacterial growth and the optimum temperature for the production of phytase from different potential phytase producing bacterial strains. A total of four strains used were originally isolated from hot springs in Malaysia which were in Labis Johor (L3) Dusun Tua Selangor (RT) Ulu Legong Kedah (A) and Ranau Sabah (B9). Nutrient Agar (NA) and modified Phytase Screening Medium (PSM) liquid media were used for the culture enrichment while optimisation was carried out through batch culture method using a shake-flask scale. Strains growth and enzyme activity were quantitatively measured at different temperatures at (30C and 37C) values. Enzyme activity was determined according to the reaction of the phytase with its substrate (sodium phytate) and expressed in units of phytase activity (U/ mL). As for the overall strain L3 (from Labis Johor) exhibit promising rate of Pi released in the media at 30Cand 37C with optimum phytase activity values of 0.2047 U/mL and 0.2195 U/mL respectively. The pH of the cultures was also measured where it shows that strains grown in cultures at 37C produced a higher phytase activity and resulting a lower reading of pH compared when grown at 30C. All around L3 strains has the lowest value of pH when cultured at 30C and 37C with the pH value of 3.62 and 2.37 respectively. From the result obtained the lower pH indicates the process of phytic acid degradation take place by the phytase in producing in organic phosphate (Pi) due to the accumulation of organic acid. Since these bacterial strains were originally taken from Hot Springs further analysis of temperature optimization using 55C and even 60C should be carried out. In the future biochemical research and molecular identification may also be carried out to identify molecular identity in the strains.

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Item Type:	Article
AGROVOC Term:	Bacteria
AGROVOC Term:	Phytase
AGROVOC Term:	Biotechnology
AGROVOC Term:	Enzymes
AGROVOC Term:	Enzyme activity
AGROVOC Term:	Cell culture
AGROVOC Term:	Soybeans
AGROVOC Term:	Culture media
AGROVOC Term:	Culture techniques
AGROVOC Term:	Growth
Depositing User:	Mr. AFANDI ABDUL MALEK
Last Modified:	24 Apr 2025 00:55
URI:	http://webagris.upm.edu.my/id/eprint/9433

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