Phenolic profile antioxidant antidiabetic DNA protection and cytotoxic activities of Allium kurtzianum

Aksoy-Sagirli P., . and Hasbal-Celikok G., . and Kocyigit M., . and Can A., . and Yilmaz-Ozden T., . and Akev N., . and Altiparmak-Ulbegi G., . Phenolic profile antioxidant antidiabetic DNA protection and cytotoxic activities of Allium kurtzianum. pp. 1000-1009. ISSN 2231-7546

Allium kurtzianum known as wild garlic in Turkey is an endemic and bulbous herb of the Amaryllidaceae family. The antioxidant activities of the aerial parts and bulbs of A. kurtzianum were determined using 2 2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging and ferric reducing antioxidant power (FRAP) assays along with the determination of total phenolic and flavonoid compounds in the extracts. Also phytochemical screening of the extracts was performed by using the LC“MS/MS method. DNA damage protection capacity of the extracts was determined using DNA nicking assay. The cytotoxic activity of the extracts was identified using different cancer cell lines. The antidiabetic activity of the extracts was analysed by evaluating inhibitory capacities on -glucosidase and -amylase. The results showed that total phenolic and flavonoid compounds of the aerial parts were higher than that of the bulbs. Of the 20 compounds quantified rutin and fumaric acid were the most abundant phenolic compounds in aerial parts and bulbs extracts respectively. The antioxidant activity of the aerial parts was greater than that of the bulbs. The extracts also dose-dependently protected pBR322 DNA against the harmful effects of hydroxyl radicals. The aerial parts of A. kurtzianum exhibited a cytotoxic effect against prostate lung and endometrial cancer cell lines whereas bulbs of A. kurtzianum did not show cytotoxic effect. However no valuable inhibitory effect on -glucosidase and -amylase was observed with either extract. In summary a methanolic extract from A. kurtzianum showed potent antioxidant and DNA protecting effects as well as anticancer activity against some cancer cells in vitro. The phenolic compounds might be responsible for these activities of the plant.

Full text available from: Official URL: http://www.ifrj.upm.edu.my/27%20(06)%202020/DONE%2...

Abstract

Allium kurtzianum known as wild garlic in Turkey is an endemic and bulbous herb of the Amaryllidaceae family. The antioxidant activities of the aerial parts and bulbs of A. kurtzianum were determined using 2 2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging and ferric reducing antioxidant power (FRAP) assays along with the determination of total phenolic and flavonoid compounds in the extracts. Also phytochemical screening of the extracts was performed by using the LC“MS/MS method. DNA damage protection capacity of the extracts was determined using DNA nicking assay. The cytotoxic activity of the extracts was identified using different cancer cell lines. The antidiabetic activity of the extracts was analysed by evaluating inhibitory capacities on -glucosidase and -amylase. The results showed that total phenolic and flavonoid compounds of the aerial parts were higher than that of the bulbs. Of the 20 compounds quantified rutin and fumaric acid were the most abundant phenolic compounds in aerial parts and bulbs extracts respectively. The antioxidant activity of the aerial parts was greater than that of the bulbs. The extracts also dose-dependently protected pBR322 DNA against the harmful effects of hydroxyl radicals. The aerial parts of A. kurtzianum exhibited a cytotoxic effect against prostate lung and endometrial cancer cell lines whereas bulbs of A. kurtzianum did not show cytotoxic effect. However no valuable inhibitory effect on -glucosidase and -amylase was observed with either extract. In summary a methanolic extract from A. kurtzianum showed potent antioxidant and DNA protecting effects as well as anticancer activity against some cancer cells in vitro. The phenolic compounds might be responsible for these activities of the plant.

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Item Type:	Article
AGROVOC Term:	Allium
AGROVOC Term:	Garlic
AGROVOC Term:	Amaryllidaceae
AGROVOC Term:	Chemical analysis (results)
AGROVOC Term:	Chromatography
AGROVOC Term:	Aerial parts
AGROVOC Term:	Bulbs
AGROVOC Term:	Phenolic compounds
AGROVOC Term:	Flavonoids
AGROVOC Term:	Alpha amylase
Depositing User:	Mr. AFANDI ABDUL MALEK
Last Modified:	24 Apr 2025 00:55
URI:	http://webagris.upm.edu.my/id/eprint/9441

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