Development of a simple paper-based colorimetric diagnostic platform for sensitive detection of Salmonella typhimurium

Mubarok A. Z., . and Schonherr M. F. P., . and Effendi F. D., . and Hsu J. L., . and Widyastuti E., . Development of a simple paper-based colorimetric diagnostic platform for sensitive detection of Salmonella typhimurium. pp. 1105-1110. ISSN 2231-7546

Salmonella has long been recognised as the most common and primary cause of food poisoning in many countries. Its detection is still primarily based on conventional microbiological culture methods which are considered as time-consuming and labour-intensive. Accordingly the development of a rapid simple and reliable detection method to detect Salmonella is crucial to maintain public health safety and security. We report herein the development of a simple and rapid paper-based platform to detect S. typhimurium. Salmonella polyclonal antibody was utilised to capture the target cells and a direct sandwich enzyme-linked immunosorbent assays (ELISA) format was then developed using an immunoglobulin G (IgG) conjugated with alkaline phosphatase (ALP) as the enzyme label. Nitro blue tetrazolium-(5-bromo-4-chloro-3-indolyl phosphate) was used as enzyme mediator system which produced a bluish-purple colour on the surface of the paper platform. The developed colour signal was then analysed using a scanner for quantitative analysis. This method was highly sensitive with a limit of detection of 6 CFU mL-1 without requiring expensive or advanced equipment. Bare eyes observation can be used for qualitative analysis thus showing the ability of this method to be used for on-site detection and in resources-limited environment.

Full text available from: Official URL: http://www.ifrj.upm.edu.my/27%20(06)%202020/DONE%2...

Abstract

Salmonella has long been recognised as the most common and primary cause of food poisoning in many countries. Its detection is still primarily based on conventional microbiological culture methods which are considered as time-consuming and labour-intensive. Accordingly the development of a rapid simple and reliable detection method to detect Salmonella is crucial to maintain public health safety and security. We report herein the development of a simple and rapid paper-based platform to detect S. typhimurium. Salmonella polyclonal antibody was utilised to capture the target cells and a direct sandwich enzyme-linked immunosorbent assays (ELISA) format was then developed using an immunoglobulin G (IgG) conjugated with alkaline phosphatase (ALP) as the enzyme label. Nitro blue tetrazolium-(5-bromo-4-chloro-3-indolyl phosphate) was used as enzyme mediator system which produced a bluish-purple colour on the surface of the paper platform. The developed colour signal was then analysed using a scanner for quantitative analysis. This method was highly sensitive with a limit of detection of 6 CFU mL-1 without requiring expensive or advanced equipment. Bare eyes observation can be used for qualitative analysis thus showing the ability of this method to be used for on-site detection and in resources-limited environment.

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Item Type:	Article
AGROVOC Term:	Salmonella typhimurium
AGROVOC Term:	Pathogenic bacteria
AGROVOC Term:	Food poisoning
AGROVOC Term:	Diagnosis
AGROVOC Term:	Colorimetry
AGROVOC Term:	Immunoassay
AGROVOC Term:	Cross-linking
AGROVOC Term:	Enzymatic activity
Depositing User:	Mr. AFANDI ABDUL MALEK
Last Modified:	24 Apr 2025 00:55
URI:	http://webagris.upm.edu.my/id/eprint/9452

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