Optimisation of hydrolysis conditions for extraction of R-phycoerythrin from Gracilaria gracilis by enzyme polysaccharidase and response surface methodology

Fleurence J., . and Morançais M., . and Nguyen-Le C. T., . and Mai T. P. C., . and Nguyen H. P. T., . and Tran T. N. L., . and Pham T. T. M., . and Dumay J., . Optimisation of hydrolysis conditions for extraction of R-phycoerythrin from Gracilaria gracilis by enzyme polysaccharidase and response surface methodology. pp. 1147-1155. ISSN 2231-7546

R-phycoerythrin has been widely used in food cosmetic pharmaceutical and analytical reagent industries. The present work reports an effective procedure for the extraction of R-phycoerythrin from Gracilaria gracilis. Firstly the algae pre-treatment is a significant stage and the highly effective extraction of R-phycoerythrin from G. gracilis was carried out with freeze-dried seaweed and grinding with liquid nitrogen. Secondly two different methods for the extraction of this pigment were tested: (i) by using the extraction solutions (tap water pure water phosphate buffer 100 mM with pH 6.5 and phosphate buffer 20 mM with pH 7.1); and (ii) by using polysaccharide-degrading enzymes (xylanase cellulase and -glucanase). Cellulase enhanced the extraction yield of R-phycoerythrin from G. gracilis (12.76 mg g-1 dw). Finally under the optimal hydrolysis conditions obtained with cellulase according to a response surface methodology study of temperature (32C) hydrolysis time (286 min) and enzyme/dry seaweed ratio (47 mg g-) maximum yield of R-phycoerythrin (15.75 mg g- dw) was obtained.

Full text available from: Official URL: http://www.ifrj.upm.edu.my/27%20(06)%202020/DONE%2...

Abstract

R-phycoerythrin has been widely used in food cosmetic pharmaceutical and analytical reagent industries. The present work reports an effective procedure for the extraction of R-phycoerythrin from Gracilaria gracilis. Firstly the algae pre-treatment is a significant stage and the highly effective extraction of R-phycoerythrin from G. gracilis was carried out with freeze-dried seaweed and grinding with liquid nitrogen. Secondly two different methods for the extraction of this pigment were tested: (i) by using the extraction solutions (tap water pure water phosphate buffer 100 mM with pH 6.5 and phosphate buffer 20 mM with pH 7.1); and (ii) by using polysaccharide-degrading enzymes (xylanase cellulase and -glucanase). Cellulase enhanced the extraction yield of R-phycoerythrin from G. gracilis (12.76 mg g-1 dw). Finally under the optimal hydrolysis conditions obtained with cellulase according to a response surface methodology study of temperature (32C) hydrolysis time (286 min) and enzyme/dry seaweed ratio (47 mg g-) maximum yield of R-phycoerythrin (15.75 mg g- dw) was obtained.

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Item Type:	Article
AGROVOC Term:	Gracilaria
AGROVOC Term:	Red algae
AGROVOC Term:	Extraction in chemical analysis
AGROVOC Term:	Methods
AGROVOC Term:	Optimization methods
AGROVOC Term:	Hydrolysis
AGROVOC Term:	Cellulase
AGROVOC Term:	Plant protein
Depositing User:	Mr. AFANDI ABDUL MALEK
Last Modified:	24 Apr 2025 00:55
URI:	http://webagris.upm.edu.my/id/eprint/9457

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