Efficacy of silvernano bacterial cellulose insole on Micrococcus sedentarius growth

Sitlaothaworn Kanchana, . and Dechkla Manussawee, . Efficacy of silvernano bacterial cellulose insole on Micrococcus sedentarius growth. pp. 2189-2200. ISSN 2231-8526

Silver nanoparticles (Ag) impregnated into bacterial cellulose (BC) by immersion in an antimicrobial concentration of 1 700 g/ml (10 mM) AgN3 solution were used to synthesize silver nanoparticle-impregnated bacterial cellulose (Ag0 BC) composites. Ag BC incorporated into insertable footpad was developed for reduction of foot odor associated with pitted keratolysis primarily caused by Micrococcus sedentarius. A scanning electron microscope (SEM) revealed the porous structure of AgBC fibers impregnated with Ag particles. The antibacterial activity of AgBC for Micrococcus sedentarius revealed a growth inhibition zone of 30 mm in diameter by disc diffusion method. The minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) of AgN3 solution against Micrococcus sedentarius were 0.42 g/ml and 0.83 g/ml respectively. The effficiency of an AgBC footpad applied to shoe samples was measured via total bacterial counts over a time period of 14 days. As expected the percentage of surviving bacteria in three different types of shoe samples was gradually decreased in the period beginning on the 2nd day. Moreover no bacterial growth was found in any shoe sample within 8“14 days. However the cytotoxicity of 5.31“1 700 g/ml AgNO· concentrations revealed 50 growth inhibition (IC‚‚) on human dermal fibroblast cells by resazurin microplate assay (REMA). Thus it can be concluded that Ag0 BC synthesized with lower doses of AgNO3 concentration ( 1 700 g/ml) may be able to apply in Ag BC footpad production for inhibition of Micrococcus sedentarius growth and prevention of foot odour in different types of footwears.

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Abstract

Silver nanoparticles (Ag) impregnated into bacterial cellulose (BC) by immersion in an antimicrobial concentration of 1 700 g/ml (10 mM) AgN3 solution were used to synthesize silver nanoparticle-impregnated bacterial cellulose (Ag0 BC) composites. Ag BC incorporated into insertable footpad was developed for reduction of foot odor associated with pitted keratolysis primarily caused by Micrococcus sedentarius. A scanning electron microscope (SEM) revealed the porous structure of AgBC fibers impregnated with Ag particles. The antibacterial activity of AgBC for Micrococcus sedentarius revealed a growth inhibition zone of 30 mm in diameter by disc diffusion method. The minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) of AgN3 solution against Micrococcus sedentarius were 0.42 g/ml and 0.83 g/ml respectively. The effficiency of an AgBC footpad applied to shoe samples was measured via total bacterial counts over a time period of 14 days. As expected the percentage of surviving bacteria in three different types of shoe samples was gradually decreased in the period beginning on the 2nd day. Moreover no bacterial growth was found in any shoe sample within 8“14 days. However the cytotoxicity of 5.31“1 700 g/ml AgNO· concentrations revealed 50 growth inhibition (IC‚‚) on human dermal fibroblast cells by resazurin microplate assay (REMA). Thus it can be concluded that Ag0 BC synthesized with lower doses of AgNO3 concentration ( 1 700 g/ml) may be able to apply in Ag BC footpad production for inhibition of Micrococcus sedentarius growth and prevention of foot odour in different types of footwears.

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Item Type:	Article
AGROVOC Term:	Micrococcus
AGROVOC Term:	Gram positive bacteria
AGROVOC Term:	Microbiological analysis
AGROVOC Term:	Cellulose
AGROVOC Term:	Silver
AGROVOC Term:	Antimicrobials
AGROVOC Term:	Growth inhibition
AGROVOC Term:	Odour
Depositing User:	Mr. AFANDI ABDUL MALEK
Last Modified:	24 Apr 2025 00:55
URI:	http://webagris.upm.edu.my/id/eprint/9461

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