Immunomodulatory activity of yogurt fortified with roselle (Hibiscus sabdariffa L.) extract

Hadi A., . and Mahfudh N., . and Solechan R. A. Z., . Immunomodulatory activity of yogurt fortified with roselle (Hibiscus sabdariffa L.) extract. pp. 255-261. ISSN 22317546

Yogurt is a probiotic food that can boost the immune system even when added with fruit extract such as roselle (Hibiscus sabdariffa). An in vivo study was carried out to prove the potency of yogurt fortified with roselle extract in enhancing the immune system using 25 male BALB/c mice. The test animals were divided into five groups namely (I) normal group (II) plain yogurt group and groups of yogurt fortified with (III) 2 (IV) 4 and (V) 8 of roselle extract respectively. The effects of these treatments were evaluated from macrophage activity using the combination of latex beads and Giemsa staining. The amounts of actively phagocytic macrophages in groups III IV and V were 89 97 and 45 respectively while the MTT assays showed that their lymphocyte proliferation activities represented by absorbance values were 0.50 0.79 and 0.68 respectively. Immunocytochemistry observation found that the secretions of interleukin-10 and interleukin-14 increased. Based on the statistical analysis there was a significant increase in the phagocytic activity of macrophages lymphocyte proliferation and secretion of IL-10 and IL-14. Overall yogurt fortified with 2 4 and 8 of roselle extract can be used as immunomodulators.

Full text available from: Official URL: http://www.ifrj.upm.edu.my/28%20(02)%202021/04%20-...

Abstract

Yogurt is a probiotic food that can boost the immune system even when added with fruit extract such as roselle (Hibiscus sabdariffa). An in vivo study was carried out to prove the potency of yogurt fortified with roselle extract in enhancing the immune system using 25 male BALB/c mice. The test animals were divided into five groups namely (I) normal group (II) plain yogurt group and groups of yogurt fortified with (III) 2 (IV) 4 and (V) 8 of roselle extract respectively. The effects of these treatments were evaluated from macrophage activity using the combination of latex beads and Giemsa staining. The amounts of actively phagocytic macrophages in groups III IV and V were 89 97 and 45 respectively while the MTT assays showed that their lymphocyte proliferation activities represented by absorbance values were 0.50 0.79 and 0.68 respectively. Immunocytochemistry observation found that the secretions of interleukin-10 and interleukin-14 increased. Based on the statistical analysis there was a significant increase in the phagocytic activity of macrophages lymphocyte proliferation and secretion of IL-10 and IL-14. Overall yogurt fortified with 2 4 and 8 of roselle extract can be used as immunomodulators.

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Item Type:	Article
AGROVOC Term:	Hibiscus sabdariffa
AGROVOC Term:	Roselle
AGROVOC Term:	Stimulant immunomodulators
AGROVOC Term:	Statistical analysis
AGROVOC Term:	Probiotics
AGROVOC Term:	Immune system
Depositing User:	Mr. AFANDI ABDUL MALEK
Last Modified:	24 Apr 2025 00:55
URI:	http://webagris.upm.edu.my/id/eprint/9493

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