Improved of ethanol production from yam bean by thermotolerant yeast Saccharomyces cerevisiae RMU Y-10 using an orthogonal array design


Citation

Wechgama Kitipong, . and Charoensopharat Kanlayani, . Improved of ethanol production from yam bean by thermotolerant yeast Saccharomyces cerevisiae RMU Y-10 using an orthogonal array design. pp. 12-22. ISSN 2672-7226

Abstract

Nitrogen and minerals are essential for the growth of yeast and in metabolic pathways of ethanol fermentation. This research examined the effects of nitrogen and added minerals on ethanol production from yam bean juice by Saccharomyces cerevisiae RMU Y-10. Three factors affecting ethanol production efficiency ” yeast extract (YE) concentrations (3 6 and 9 g/L) diammonium hydrogen phosphate (DAP) concentrations (0.25 0.50 and 0.75 g/L) and MgSO‚„ concentrations (0.5 1.0 and 1.5 g/l) ” were investigated using a L‚ (3�´) Orthogonal array design. Fermentation was performed at 37C and YE showed the greatest influence on ethanol concentration followed by DAP and MgSO‚„. The highest ethanol concentration of 51.2 g/L with yield (Yp/s) and productivity (Qp) values of 0.51 and 0.71 g/L.h respectively was obtained under the optimal conditions of 9 g/L YE 0.75 g/L DAP and 0.5 g/L MgSO‚„. The ethanol concentration obtained under the optimum conditions was about 30 higher than those obtained in control sets using YM medium or yam bean juice without nutrients added to the substrates.


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Abstract

Nitrogen and minerals are essential for the growth of yeast and in metabolic pathways of ethanol fermentation. This research examined the effects of nitrogen and added minerals on ethanol production from yam bean juice by Saccharomyces cerevisiae RMU Y-10. Three factors affecting ethanol production efficiency ” yeast extract (YE) concentrations (3 6 and 9 g/L) diammonium hydrogen phosphate (DAP) concentrations (0.25 0.50 and 0.75 g/L) and MgSO‚„ concentrations (0.5 1.0 and 1.5 g/l) ” were investigated using a L‚ (3�´) Orthogonal array design. Fermentation was performed at 37C and YE showed the greatest influence on ethanol concentration followed by DAP and MgSO‚„. The highest ethanol concentration of 51.2 g/L with yield (Yp/s) and productivity (Qp) values of 0.51 and 0.71 g/L.h respectively was obtained under the optimal conditions of 9 g/L YE 0.75 g/L DAP and 0.5 g/L MgSO‚„. The ethanol concentration obtained under the optimum conditions was about 30 higher than those obtained in control sets using YM medium or yam bean juice without nutrients added to the substrates.

Additional Metadata

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Item Type: Article
AGROVOC Term: Yam beans
AGROVOC Term: Pachyrhizus
AGROVOC Term: Yeast extracts
AGROVOC Term: Saccharomyces cerevisiae
AGROVOC Term: Fermentation
AGROVOC Term: Ethanol
AGROVOC Term: Production
Depositing User: Mr. AFANDI ABDUL MALEK
Last Modified: 24 Apr 2025 00:55
URI: http://webagris.upm.edu.my/id/eprint/9625

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