Response of auxins and cytokinins on citrus suhuiensis adventitious shoot culture initiation and growth

Noor Illi Mohamad Puad and Wan Majidah Wan Zulkifli and Nur Alia M. Fathil and Azura Amid (2023) Response of auxins and cytokinins on citrus suhuiensis adventitious shoot culture initiation and growth. Journal of Agrobiotechnology (Malaysia), 14. pp. 21-31. ISSN 2180-1983

Bacterial and viral diseases are the common problems hampering the vast majority of citrus plants which resulted in a decrease of citrus trees development and production yield. While the usage of chemicals to eliminate internal pathogens is harmful to the ecosystem, plant tissue culture is another alternative to develop disease-free plants based on the defined physical and chemical conditions under an aseptic environment. This study aimed to initiate Citrus suhuiensis (C. suhuiensis)adventitious shoot culture specifically in response towards different types of plant growth regulators (PGRs). The effects of explants and PGRs were evaluated on the shoot growth within 35 days of culture. C. suhuiensis shoot cultures were induced from three different explants which are leaf, callus and seeds on the solid Murashige and Skoog (MS) medium containing different combinations of PGRs which are auxins i.e. 1-naphthylacetic acid (NAA) or indole butyric acid (IBA) at 0.5 mg/L, respectively with cytokinins i.e. 6-benzylaminopurine(BAP) or kinetin (KN) at various concentrations (0.5 -4.0 mg/L). Based on the results, the earliest shoot emergence from the cotyledon can be observed after the 8thday of inoculation for PGRs combination of 0.5 mg/L IBA with 2.0 mg/L, 3.0 mg/L KN and 3.0 mg/L BAP, respectively. Meanwhile, based on the ANOVA analysis (p-value < 0.05), the most significant PGRs combination for the establishment of C. suhuiensis shoot culture is IBA and KN followed by the treatment of NAA and KN. The findings of this study can serve as a basis for future investigation of micropropagation of shoot culture and cultivation of C. suhuiensis plant

Full text available from: Official URL: https://journal.unisza.edu.my/agrobiotechnology/in...

Abstract

Bacterial and viral diseases are the common problems hampering the vast majority of citrus plants which resulted in a decrease of citrus trees development and production yield. While the usage of chemicals to eliminate internal pathogens is harmful to the ecosystem, plant tissue culture is another alternative to develop disease-free plants based on the defined physical and chemical conditions under an aseptic environment. This study aimed to initiate Citrus suhuiensis (C. suhuiensis)adventitious shoot culture specifically in response towards different types of plant growth regulators (PGRs). The effects of explants and PGRs were evaluated on the shoot growth within 35 days of culture. C. suhuiensis shoot cultures were induced from three different explants which are leaf, callus and seeds on the solid Murashige and Skoog (MS) medium containing different combinations of PGRs which are auxins i.e. 1-naphthylacetic acid (NAA) or indole butyric acid (IBA) at 0.5 mg/L, respectively with cytokinins i.e. 6-benzylaminopurine(BAP) or kinetin (KN) at various concentrations (0.5 -4.0 mg/L). Based on the results, the earliest shoot emergence from the cotyledon can be observed after the 8thday of inoculation for PGRs combination of 0.5 mg/L IBA with 2.0 mg/L, 3.0 mg/L KN and 3.0 mg/L BAP, respectively. Meanwhile, based on the ANOVA analysis (p-value < 0.05), the most significant PGRs combination for the establishment of C. suhuiensis shoot culture is IBA and KN followed by the treatment of NAA and KN. The findings of this study can serve as a basis for future investigation of micropropagation of shoot culture and cultivation of C. suhuiensis plant

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Item Type:	Article
AGROVOC Term:	Citrus
AGROVOC Term:	plant growth regulators > plant growth regulators Prefer using plant growth substancesplant growth substances
AGROVOC Term:	micropropagation
AGROVOC Term:	statistical analysis > statistical analysis Prefer using statistical methodsstatistical methods
AGROVOC Term:	cytokinins
AGROVOC Term:	plant tissues
AGROVOC Term:	shoot tip culture > shoot tip culture Prefer using meristem culturemeristem culture
Geographical Term:	Malaysia
Uncontrolled Keywords:	Citrus suhuiensis
Depositing User:	Nor Hasnita Abdul Samat
Date Deposited:	27 Aug 2024 15:17
Last Modified:	27 Aug 2024 15:17
URI:	http://webagris.upm.edu.my/id/eprint/964

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