Citation
Tan Chin Yee, . and Voon Chun Ping, . and Maizan Haiji Mohamed, . and Shazrul Fazry, . and Law Douglas, . and Nik Marzuki Sidik, . and Herryawan Ryadi Eziwar Dyari, . Use of amplified fragment length polymorphism and sequence characterized amplified region marker for identifying the sex of the Oxyeleotris marmorata. pp. 107-115.
Abstract
There is a huge demand for the Oxyeleotris marmorata especially in Asian markets. However farmers are unable to provide a constant supply of this fish to meet the demand which is estimated to be round 100 metric tonnes per annum. One of the reasons that are hindering the supply is the low success rate of O. marmorata breeding programs. These breeding programs rely on many factors for their success one of which is the use of genuine male and female adults although determining these could be a daunting task. This research was carried out in an attempt to determine a sex marker for the O. marmorata using the amplified fragment length polymorphism (AFLP) method. Of the 3030 AFLP primer mixtures screened the E-TAA and M-CTT primer pair had an amplified 600 bp marker that was specific to the female. This 600 bp AFLP marker was later used to design a 464 bp sequence characterized amplified region (SCAR) marker. Thus it has been suggested that the SCAR marker obtained has the potential to be used for the sexual identification of the O. marmorata at the juvenile stage thereby enabling them to be used in breeding programs.
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Abstract
There is a huge demand for the Oxyeleotris marmorata especially in Asian markets. However farmers are unable to provide a constant supply of this fish to meet the demand which is estimated to be round 100 metric tonnes per annum. One of the reasons that are hindering the supply is the low success rate of O. marmorata breeding programs. These breeding programs rely on many factors for their success one of which is the use of genuine male and female adults although determining these could be a daunting task. This research was carried out in an attempt to determine a sex marker for the O. marmorata using the amplified fragment length polymorphism (AFLP) method. Of the 3030 AFLP primer mixtures screened the E-TAA and M-CTT primer pair had an amplified 600 bp marker that was specific to the female. This 600 bp AFLP marker was later used to design a 464 bp sequence characterized amplified region (SCAR) marker. Thus it has been suggested that the SCAR marker obtained has the potential to be used for the sexual identification of the O. marmorata at the juvenile stage thereby enabling them to be used in breeding programs.
Additional Metadata
| Item Type: | Article |
|---|---|
| AGROVOC Term: | Oxyeleotris marmorata |
| AGROVOC Term: | Fishes |
| AGROVOC Term: | Aquatic animals |
| AGROVOC Term: | Animal breeding |
| AGROVOC Term: | Sex determination |
| AGROVOC Term: | Isolation |
| AGROVOC Term: | Juveniles |
| AGROVOC Term: | Genetic markers |
| AGROVOC Term: | DNA sequence |
| AGROVOC Term: | Polymorphism |
| Depositing User: | Mr. AFANDI ABDUL MALEK |
| Last Modified: | 24 Apr 2025 00:55 |
| URI: | http://webagris.upm.edu.my/id/eprint/9664 |
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