Molecular characterisation of the GdhA� derivative of Pasteurella multocida B:2

Teerasak E-kobon, . and Mohd. Zamri Saad, . and Farahani Muhammad Azam, . and Raha Abdul Rahim, . and Pramote Chumnanpuen, . and Sarah Othman, . Molecular characterisation of the GdhA� derivative of Pasteurella multocida B:2. pp. 171-192. ISSN 1511-3701

Pasteurella multocida B:2 is an important veterinary pathogen causing fatal and acute haemorrhagic septicaemia (HS) in bovine. A live vaccine candidate P. multocida B:2 GDH7 was reported to enable protection in cattle and buffaloes via intranasal (i. n.) administration. This potential vaccine was also reported to be self-transmitted from the vaccinated animal to the free-ranging animals allowing wider vaccination coverage. Prior to commercialisation this potential vaccine requires further characterisation in accordance with the authoritative guidelines from the World Organisation for Animal Health (OIE). Hence in this study the potential vaccine strain P. multocida B:2 GDH7 and the virulent parent strain were characterized through genomic and proteomic profiling. A crucial first step was to develop a sensitive yet simple and robust identification test to differentiate both strains which has been achieved by the development of a precise yet straightforward PCR method. In genomic profiling Repetitive Extragenic Palindromic sequence-PCR (REP-PCR was manipulated and both strains have a different display of genomic DNA band patterns. Some of the major OMPs were observed and prominent immunogens of P. multocida OmpA and OmpH were observed to be expressed differently between these strains through SDS-PAGE analysis. In conclusion a reproducible PCR detection method has enabled differentiation of both strains. Further characterisation of these strains shows a significantly different profile through genomic and proteomic profiling.

Full text available from: Official URL: http://www.pertanika.upm.edu.my/resources/files/Pe...

Abstract

Pasteurella multocida B:2 is an important veterinary pathogen causing fatal and acute haemorrhagic septicaemia (HS) in bovine. A live vaccine candidate P. multocida B:2 GDH7 was reported to enable protection in cattle and buffaloes via intranasal (i. n.) administration. This potential vaccine was also reported to be self-transmitted from the vaccinated animal to the free-ranging animals allowing wider vaccination coverage. Prior to commercialisation this potential vaccine requires further characterisation in accordance with the authoritative guidelines from the World Organisation for Animal Health (OIE). Hence in this study the potential vaccine strain P. multocida B:2 GDH7 and the virulent parent strain were characterized through genomic and proteomic profiling. A crucial first step was to develop a sensitive yet simple and robust identification test to differentiate both strains which has been achieved by the development of a precise yet straightforward PCR method. In genomic profiling Repetitive Extragenic Palindromic sequence-PCR (REP-PCR was manipulated and both strains have a different display of genomic DNA band patterns. Some of the major OMPs were observed and prominent immunogens of P. multocida OmpA and OmpH were observed to be expressed differently between these strains through SDS-PAGE analysis. In conclusion a reproducible PCR detection method has enabled differentiation of both strains. Further characterisation of these strains shows a significantly different profile through genomic and proteomic profiling.

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Item Type:	Article
AGROVOC Term:	Cattle
AGROVOC Term:	Buffaloes (bubalus)
AGROVOC Term:	Bacterial diseases
AGROVOC Term:	Disease control
AGROVOC Term:	Vaccination
AGROVOC Term:	PCR
AGROVOC Term:	Pasteurella multocida
AGROVOC Term:	Live vaccines
AGROVOC Term:	Vaccines
AGROVOC Term:	Genomes
Depositing User:	Mr. AFANDI ABDUL MALEK
Last Modified:	24 Apr 2025 00:55
URI:	http://webagris.upm.edu.my/id/eprint/9668

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