Catechin content and free radical scavenging activity of Camellia sinensis twig extracts


Citation

Hung Y. C., . and Hsiao Y. H., . and Hsieh J. F., . Catechin content and free radical scavenging activity of Camellia sinensis twig extracts. pp. 248-254. ISSN 22317546

Abstract

The catechin content and antioxidant properties of various Camellia sinensis twig extracts including a water extract (WE) 10 ethanol extract (10 EE) 50 ethanol extract (50 EE) and 95 ethanol extract (95 EE) were investigated. The 50 EE had the highest total phenolic content (161.3 8.5 mg gallic acid equivalents/L) and total flavonoid content (278.9 12.2 mg quercetin equivalents/L). High-performance liquid chromatography analysis suggested that epigallocatechin gallate and epigallocatechin were the predominant catechins in the twig extracts. The relative concentrations of six catechins isolated from the extracts were: 50 EE 10 EE WE 95 EE. The 50 EE showed free radical-scavenging activity. The concentration of dry matter of 50 EE required to scavenge 50 of ABTS radicals was 102.8 4.2 g/mL. These results suggest that 50 EE can potentially be used as a source of catechins.


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Abstract

The catechin content and antioxidant properties of various Camellia sinensis twig extracts including a water extract (WE) 10 ethanol extract (10 EE) 50 ethanol extract (50 EE) and 95 ethanol extract (95 EE) were investigated. The 50 EE had the highest total phenolic content (161.3 8.5 mg gallic acid equivalents/L) and total flavonoid content (278.9 12.2 mg quercetin equivalents/L). High-performance liquid chromatography analysis suggested that epigallocatechin gallate and epigallocatechin were the predominant catechins in the twig extracts. The relative concentrations of six catechins isolated from the extracts were: 50 EE 10 EE WE 95 EE. The 50 EE showed free radical-scavenging activity. The concentration of dry matter of 50 EE required to scavenge 50 of ABTS radicals was 102.8 4.2 g/mL. These results suggest that 50 EE can potentially be used as a source of catechins.

Additional Metadata

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Item Type: Article
AGROVOC Term: Tea
AGROVOC Term: Camellia sinensis
AGROVOC Term: Catechin
AGROVOC Term: Antioxidants
AGROVOC Term: Chromatography
AGROVOC Term: Statistical analysis
AGROVOC Term: Sampling
AGROVOC Term: Food industry
AGROVOC Term: Polyphenols
AGROVOC Term: Antioxidants
Depositing User: Mr. AFANDI ABDUL MALEK
Last Modified: 24 Apr 2025 00:55
URI: http://webagris.upm.edu.my/id/eprint/9830

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