In silico characterization of the structure of genes and proteins related to -carotene degradation in Musa acuminata DH-Pahang and Musa balbisiana Pisang Klutuk Wulung

Dwivany Fenny Martha, . and Sukriandi Nisrina, . and Meitha Karlia, . and Brotosudarmo Tatas H. P., . In silico characterization of the structure of genes and proteins related to -carotene degradation in Musa acuminata DH-Pahang and Musa balbisiana Pisang Klutuk Wulung. pp. 429-447. ISSN 1511-3701

-carotene is an important nutritional content in banana. However its lifetime depends on the enzymes controlling its conversion into strigolactone. To understand the involved enzymes activity which are -carotene isomerase (D27) carotenoid cleavage dioxygenase 7 (CCD7) and CCD8 would be the key to manipulate the rate of -carotene degradation. In this research the structure of genes and proteins of the D27 CCD7 and CCD8 from Musa acuminata were characterized. DH-Pahang and Musa balbisiana Pisang Klutuk Wulung (PKW). The corresponding sequence of genes from both species were aligned to determine similarity and intron/exon positions. Domains and motifs in the sequences of putative proteins of D27 CCD7 and CCD8 were also identified. It was found that D27 CCD7 and CCD8 genes in DH-Pahang and PKW comprise of various nucleotide sequence length putative proteins and numbers and length of exons and introns. However the putative proteins possess the same domains: DUF4033 (domain of unknown function) in D27 and RPE65 (retinal pigment epithelium) in CCD7 and CCD8. Phylogenetic trees showed that D27 CCD7 and CCD8 proteins from DH-Pahang and PKW are conserved and clustered in the same clades with the same proteins of monocot plants. Hence the results could be useful for future research in optimizing -carotene content in banana.

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Abstract

-carotene is an important nutritional content in banana. However its lifetime depends on the enzymes controlling its conversion into strigolactone. To understand the involved enzymes activity which are -carotene isomerase (D27) carotenoid cleavage dioxygenase 7 (CCD7) and CCD8 would be the key to manipulate the rate of -carotene degradation. In this research the structure of genes and proteins of the D27 CCD7 and CCD8 from Musa acuminata were characterized. DH-Pahang and Musa balbisiana Pisang Klutuk Wulung (PKW). The corresponding sequence of genes from both species were aligned to determine similarity and intron/exon positions. Domains and motifs in the sequences of putative proteins of D27 CCD7 and CCD8 were also identified. It was found that D27 CCD7 and CCD8 genes in DH-Pahang and PKW comprise of various nucleotide sequence length putative proteins and numbers and length of exons and introns. However the putative proteins possess the same domains: DUF4033 (domain of unknown function) in D27 and RPE65 (retinal pigment epithelium) in CCD7 and CCD8. Phylogenetic trees showed that D27 CCD7 and CCD8 proteins from DH-Pahang and PKW are conserved and clustered in the same clades with the same proteins of monocot plants. Hence the results could be useful for future research in optimizing -carotene content in banana.

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Item Type:	Article
AGROVOC Term:	Carotenes
AGROVOC Term:	Musa (bananas)
AGROVOC Term:	Genes
AGROVOC Term:	analysis
AGROVOC Term:	Genomes
AGROVOC Term:	Carotenes
AGROVOC Term:	Plant structure
AGROVOC Term:	plant genetics
Depositing User:	Mr. AFANDI ABDUL MALEK
Last Modified:	24 Apr 2025 00:55
URI:	http://webagris.upm.edu.my/id/eprint/9847

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