In vitro antidiabetic property and phytochemical profiling of Mangifera odorata pulp using UHPLC-ESI-Orbitrap-MS/MS

R. Karim, . and N. S. Ramli, . and M. S. Pak Dek, . and N. F. Lasano, . and A. Abdul Hamid, . and F. Abas, . In vitro antidiabetic property and phytochemical profiling of Mangifera odorata pulp using UHPLC-ESI-Orbitrap-MS/MS. pp. 366-376. ISSN 22317546

Mangifera odorata or known as kuini� in Malay is considered an underutilised climacteric tropical fruit. Recent studies had suggested that the by-products of M. odorata may serve as functional food ingredients based on their nutritional values and biological activities. Thus the present work was aimed to determine the nutritional composition antidiabetic properties through -glucosidase and -amylase inhibition assays and phytochemical profiling of M. odorata pulp using ultra-high-performance liquid chromatography-electrospray ionization orbitrap tandem mass spectrometry (UHPLC-ESI-Orbitrap-MS/MS). Proximate analyses found that M. odorata pulp contained high amounts of soluble fibre and moisture. The main sugar in the pulp was identified as sucrose while -carotene potassium calcium iron and zinc were the major vitamins and minerals. The extraction of the sample in water acetone methanol and ethanol were performed for the determination of antidiabetic assay. The results showed that 60 of ethanolic extract had the highest -amylase inhibitory activity among the four extraction solvents. The findings revealed high levels of mangiferin in the active extract which may be responsible for the -amylase inhibition activity thus contributing to lower post-prandial blood glucose. The present work provides evidence on the nutritional benefits of M. odorata pulp as well as the potential use of its ethanolic extract as an -amylase inhibitor.

Full text available from: Official URL: http://www.ifrj.upm.edu.my/28%20(02)%202021/18%20-...

Abstract

Mangifera odorata or known as kuini� in Malay is considered an underutilised climacteric tropical fruit. Recent studies had suggested that the by-products of M. odorata may serve as functional food ingredients based on their nutritional values and biological activities. Thus the present work was aimed to determine the nutritional composition antidiabetic properties through -glucosidase and -amylase inhibition assays and phytochemical profiling of M. odorata pulp using ultra-high-performance liquid chromatography-electrospray ionization orbitrap tandem mass spectrometry (UHPLC-ESI-Orbitrap-MS/MS). Proximate analyses found that M. odorata pulp contained high amounts of soluble fibre and moisture. The main sugar in the pulp was identified as sucrose while -carotene potassium calcium iron and zinc were the major vitamins and minerals. The extraction of the sample in water acetone methanol and ethanol were performed for the determination of antidiabetic assay. The results showed that 60 of ethanolic extract had the highest -amylase inhibitory activity among the four extraction solvents. The findings revealed high levels of mangiferin in the active extract which may be responsible for the -amylase inhibition activity thus contributing to lower post-prandial blood glucose. The present work provides evidence on the nutritional benefits of M. odorata pulp as well as the potential use of its ethanolic extract as an -amylase inhibitor.

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Item Type:	Article
AGROVOC Term:	Mangifera
AGROVOC Term:	Alpha glucosidase
AGROVOC Term:	Alpha amylase
AGROVOC Term:	Diabetes mellitus
AGROVOC Term:	Sampling
AGROVOC Term:	Statistical analysis
AGROVOC Term:	Analysis of variance
AGROVOC Term:	Mineral content
AGROVOC Term:	Nutritional value
AGROVOC Term:	Dietary fibres
Depositing User:	Mr. AFANDI ABDUL MALEK
Last Modified:	24 Apr 2025 00:55
URI:	http://webagris.upm.edu.my/id/eprint/9878

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