Development of genic SSR markers from transcriptome sequences of Baeckea frutescens (cucur atap) for genetic diversity assessment

Kit Kevin Siong Ng, . and Chai Ting Lee, . and Nur Nabilah Alias, . and Lee Hong Tnah, . and Nurul Farhanah Zakaria, . and Soon Leong Lee, . and Chin Hong Ng, . and Norlia Basherudin, . Development of genic SSR markers from transcriptome sequences of Baeckea frutescens (cucur atap) for genetic diversity assessment. pp. 15-25. ISSN 1985-0484

Baeckea frutescens or Cucur Atap is a medicinal plant that has been traditionally used as herbal medicine and has great potential for pharmaceutical application due to its biological properties. The increasing demand for B. frutescens herbal medicinal products has led to its over-exploitation. Therefore it is crucial to generate baseline genetic information in order to formulate conservation and breeding programmes for this species. In this study a total of 26 polymorphic simple sequence repeat (SSR) markers have been developed in B. frutescens based on the leaf transcriptome sequence data obtained via next generation sequencing (NGS) technology. These markers were evaluated using 32 individuals from Forest Research Institute Malaysia Research Station in Setiu Terengganu. The mean number of alleles was two and the average expected (HE) and observed heterozygosity (HO) were 0.484 and 0.900 respectively. Cluster analysis revealed high genetic similarity among the individuals whereby many of them were of the same clones. It is essential to assess more individuals from other populations for better understanding on the genetic diversity of this useful medicinal plant.

Full text available from: Official URL: https://www.jtpp.org.my/volume/13_1/vol13_1_articl...

Abstract

Baeckea frutescens or Cucur Atap is a medicinal plant that has been traditionally used as herbal medicine and has great potential for pharmaceutical application due to its biological properties. The increasing demand for B. frutescens herbal medicinal products has led to its over-exploitation. Therefore it is crucial to generate baseline genetic information in order to formulate conservation and breeding programmes for this species. In this study a total of 26 polymorphic simple sequence repeat (SSR) markers have been developed in B. frutescens based on the leaf transcriptome sequence data obtained via next generation sequencing (NGS) technology. These markers were evaluated using 32 individuals from Forest Research Institute Malaysia Research Station in Setiu Terengganu. The mean number of alleles was two and the average expected (HE) and observed heterozygosity (HO) were 0.484 and 0.900 respectively. Cluster analysis revealed high genetic similarity among the individuals whereby many of them were of the same clones. It is essential to assess more individuals from other populations for better understanding on the genetic diversity of this useful medicinal plant.

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Item Type:	Article
AGROVOC Term:	Medicinal plants
AGROVOC Term:	Myrtaceae
AGROVOC Term:	Gene sequence
AGROVOC Term:	Leaf extracts
AGROVOC Term:	Random amplified polymorphic DNA
AGROVOC Term:	Microsatellites
AGROVOC Term:	DNA sequence
AGROVOC Term:	Genetic diversity as resource
AGROVOC Term:	Germplasm collections
AGROVOC Term:	Nature conservation
Depositing User:	Mr. AFANDI ABDUL MALEK
Last Modified:	24 Apr 2025 00:55
URI:	http://webagris.upm.edu.my/id/eprint/9884

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