Genetic diversity and population structure of endangered catfish Rita rita (Hamilton 1822) revealed by heterologous DNA microsatellite markers

Rahman Mohammad Matiur, . and Salam Md. Abdus, . and Mollah Md. Fazlul Awal, . and Ali Muhammad Forhad, . and Sarder Md. Rafiqul Islam, . Genetic diversity and population structure of endangered catfish Rita rita (Hamilton 1822) revealed by heterologous DNA microsatellite markers. pp. 181-194. ISSN 0116-6514

Genetic information is essential for conservation and future aquaculture development of the endangered catfish Rita rita (Hamilton 1822). Two hundred catfish R. rita 50 from four rivers the Old Brahmaputra Jamuna Meghna and Kangsa were collected and analysed to evaluate the genetic diversity and population structure using five microsatellite primers (Cba06-KUL Cba08-KUL Cba09-KUL Phy03-KUL and Phy07-KUL). Four of the five amplified loci were found polymorphic (P95) in all the populations and 46 alleles were recorded with 9 to 14 alleles per locus. Differences were observed in the total number of alleles ranging from 41 to 44 effective number of alleles from 29.96 to 37.46 observed heterozygosity from 0.57 to 0.76 Shannons information index from 2.09 to 2.30 and polymorphic information content from 0.84 to 0.88 among the four populations. Results exposed the highest levels of genetic diversity in the Meghna population while the lowest in the Kangsa population of R. rita. All the populations were significantly deviated (P 0.001) from the Hardy-Weinberg equilibrium for all the loci. Neis genetic distance between populations ranged 0.007 to 0.017 with low overall genetic difference FST 0.011 and high gene flow Nm 24.333 indicating that R. rita populations were not subdivided. This study revealed a high level of gene diversity with deficiency in genetic heterogeneity in all the populations of R. rita emphasising natural management conservation and rehabilitation measures of this species.

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Abstract

Genetic information is essential for conservation and future aquaculture development of the endangered catfish Rita rita (Hamilton 1822). Two hundred catfish R. rita 50 from four rivers the Old Brahmaputra Jamuna Meghna and Kangsa were collected and analysed to evaluate the genetic diversity and population structure using five microsatellite primers (Cba06-KUL Cba08-KUL Cba09-KUL Phy03-KUL and Phy07-KUL). Four of the five amplified loci were found polymorphic (P95) in all the populations and 46 alleles were recorded with 9 to 14 alleles per locus. Differences were observed in the total number of alleles ranging from 41 to 44 effective number of alleles from 29.96 to 37.46 observed heterozygosity from 0.57 to 0.76 Shannons information index from 2.09 to 2.30 and polymorphic information content from 0.84 to 0.88 among the four populations. Results exposed the highest levels of genetic diversity in the Meghna population while the lowest in the Kangsa population of R. rita. All the populations were significantly deviated (P 0.001) from the Hardy-Weinberg equilibrium for all the loci. Neis genetic distance between populations ranged 0.007 to 0.017 with low overall genetic difference FST 0.011 and high gene flow Nm 24.333 indicating that R. rita populations were not subdivided. This study revealed a high level of gene diversity with deficiency in genetic heterogeneity in all the populations of R. rita emphasising natural management conservation and rehabilitation measures of this species.

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Item Type:	Article
AGROVOC Term:	Rita rita
AGROVOC Term:	Bagridae
AGROVOC Term:	Genetic variation
AGROVOC Term:	Aquaculture
AGROVOC Term:	Sampling
AGROVOC Term:	Statistical analysis
AGROVOC Term:	Microsatellites
AGROVOC Term:	Fisheries data
AGROVOC Term:	Preservation
AGROVOC Term:	Polymorphism
Depositing User:	Mr. AFANDI ABDUL MALEK
Last Modified:	24 Apr 2025 00:55
URI:	http://webagris.upm.edu.my/id/eprint/9896

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