Analysis of metabolites of nitrofuran antibiotics in animal-derived food by UPLC-MS/MS

Xu Z., . and Luo Z., . and Zhang W., . and Lv Z., . and Lu J., . and Chen A., . Analysis of metabolites of nitrofuran antibiotics in animal-derived food by UPLC-MS/MS. pp. 467-478. ISSN 22317546

An ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method was used for the simultaneous detection of four metabolites of nitrofuran (NF) antibiotics in eight animal-derived foods namely porcine muscle chicken fish duck pork liver crab shrimp and egg. Briefly the sample was first acid-hydrolysed derivatised and extracted by ethyl acetate. The extract was then analysed by UPLC-MS/MS. Later sample pre-treatment and UPLC-MS/MS conditions were optimised. The results showed that the method had good linearity over the range of 0.550 gkg-. The average recoveries were 80.3119.0 and the relative standard deviations (RSDs) were 8.1 and 10.9 for intra-assay and inter-assay precision respectively. The limits of detection (LODs) for 3-amino-2-oxazolidinone (AOZ) semicarbazide (SEM) 5-morpholino-3-amino-2-oxazolidone (AMOZ) and 1-amino-hydantoin (AHD) were 0.1 0.2 0.2 and 0.40.5 gkg- respectively and the limits of quantification (LOQs) for AOZ SEM AMOZ and AHD were 0.4 0.5 0.5 and 0.81.0 gkg- respectively. The proposed method was used to detect NF residues in 100 animal-derived food samples and quality control samples. The results were close to those detected by the China national standard method GB/T 20752-2006 and the results of quality control samples were within the detectable ranges. The results can provide a theoretical basis for the detection of NF residues in different kinds of animal-derived foods.

Full text available from: Official URL: http://www.ifrj.upm.edu.my/28%20(03)%202021/06%20-...

Abstract

An ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method was used for the simultaneous detection of four metabolites of nitrofuran (NF) antibiotics in eight animal-derived foods namely porcine muscle chicken fish duck pork liver crab shrimp and egg. Briefly the sample was first acid-hydrolysed derivatised and extracted by ethyl acetate. The extract was then analysed by UPLC-MS/MS. Later sample pre-treatment and UPLC-MS/MS conditions were optimised. The results showed that the method had good linearity over the range of 0.550 gkg-. The average recoveries were 80.3119.0 and the relative standard deviations (RSDs) were 8.1 and 10.9 for intra-assay and inter-assay precision respectively. The limits of detection (LODs) for 3-amino-2-oxazolidinone (AOZ) semicarbazide (SEM) 5-morpholino-3-amino-2-oxazolidone (AMOZ) and 1-amino-hydantoin (AHD) were 0.1 0.2 0.2 and 0.40.5 gkg- respectively and the limits of quantification (LOQs) for AOZ SEM AMOZ and AHD were 0.4 0.5 0.5 and 0.81.0 gkg- respectively. The proposed method was used to detect NF residues in 100 animal-derived food samples and quality control samples. The results were close to those detected by the China national standard method GB/T 20752-2006 and the results of quality control samples were within the detectable ranges. The results can provide a theoretical basis for the detection of NF residues in different kinds of animal-derived foods.

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Item Type:	Article
AGROVOC Term:	Metabolites
AGROVOC Term:	Antibiotics
AGROVOC Term:	Nitrofurans
AGROVOC Term:	Sampling
AGROVOC Term:	Analytical methods
AGROVOC Term:	Quality controls
AGROVOC Term:	Mass spectrometry
Depositing User:	Mr. AFANDI ABDUL MALEK
Last Modified:	24 Apr 2025 00:55
URI:	http://webagris.upm.edu.my/id/eprint/9905

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