Detection of plasmid mediated colistin resistant (mcr-1) gene in Salmonella spp. isolated from chicken

Siti Norhanani R., . and Khoo E., . and Nafizah M., . and Normah M. A., . and Roseliza R., . and Nurul Syafiqah Z., . and Mohammad Fhitri S., . and Faizah Hanim M. S., . Detection of plasmid mediated colistin resistant (mcr-1) gene in Salmonella spp. isolated from chicken. pp. 32-39. ISSN 2180-3897

The emergence of multidrug resistant (MDR) microbes is a major threat worldwide and it has become worse with the emergence of resistance towards colistin antibiotic. Presence of plasmid mediated colistin resistant gene (mcr-1) in bacteria contributes much to the spread of colistin resistant among Enterobacteriaceae such as Escherichia.coli and Salmonella spp. Since the first description of mcr-1 gene in E. coli from livestock in 2015 numerous reports had revealed a worldwide spread of mcr-1 gene among foodborne pathogen. In Malaysia there is a lack of data on detection of mcr-1 gene among Salmonella strains in food producing animals. Hence this paper focuses on the detection of mcr-1 gene in Salmonella spp. isolated from chickens submitted to the Veterinary Research Institute (VRI) using published primers. A total of 284 salmonella isolates retrieved from monitoring and surveillance cases submitted to VRI from year 2016 and 2017 were used in this study. 3.17 (9/284) of the isolates were found to carry the mcr-1 gene. This finding supports the existence of mcr-1 gene in Salmonella spp. from chickens in the country. Antimicrobial resistance problems become complex when the bacterial strain carrying mcr-1 gene become highly resistant to colistin the last choice of antibiotic mainly used for the treatment against Gram-negative bacterial infection both in human and animals. Inter-agency collaboration to gather baseline data on colistin usage is crucial to assess the issue and help draw guidelines to reduce the impact of colistin resistance in Malaysia

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Abstract

The emergence of multidrug resistant (MDR) microbes is a major threat worldwide and it has become worse with the emergence of resistance towards colistin antibiotic. Presence of plasmid mediated colistin resistant gene (mcr-1) in bacteria contributes much to the spread of colistin resistant among Enterobacteriaceae such as Escherichia.coli and Salmonella spp. Since the first description of mcr-1 gene in E. coli from livestock in 2015 numerous reports had revealed a worldwide spread of mcr-1 gene among foodborne pathogen. In Malaysia there is a lack of data on detection of mcr-1 gene among Salmonella strains in food producing animals. Hence this paper focuses on the detection of mcr-1 gene in Salmonella spp. isolated from chickens submitted to the Veterinary Research Institute (VRI) using published primers. A total of 284 salmonella isolates retrieved from monitoring and surveillance cases submitted to VRI from year 2016 and 2017 were used in this study. 3.17 (9/284) of the isolates were found to carry the mcr-1 gene. This finding supports the existence of mcr-1 gene in Salmonella spp. from chickens in the country. Antimicrobial resistance problems become complex when the bacterial strain carrying mcr-1 gene become highly resistant to colistin the last choice of antibiotic mainly used for the treatment against Gram-negative bacterial infection both in human and animals. Inter-agency collaboration to gather baseline data on colistin usage is crucial to assess the issue and help draw guidelines to reduce the impact of colistin resistance in Malaysia

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Item Type:	Article
AGROVOC Term:	Salmonella
AGROVOC Term:	Isolation of microorganisms
AGROVOC Term:	Agglutination tests
AGROVOC Term:	Chickens
AGROVOC Term:	Polymerase chain reaction
AGROVOC Term:	DNA
AGROVOC Term:	Plasmids
AGROVOC Term:	Genes
AGROVOC Term:	Colistin
AGROVOC Term:	Serotypes
Depositing User:	Mr. AFANDI ABDUL MALEK
Last Modified:	24 Apr 2025 00:55
URI:	http://webagris.upm.edu.my/id/eprint/9922

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