Characterisation of Stenotrophomonas maltophilia isolated from marine sponge producing salt tolerant proteases

Nurulhuda Mohd Jailania, . and Nurul Sherina Muzainia, . and Tengku Haziyamin Tengku Abdul Hamid, . Characterisation of Stenotrophomonas maltophilia isolated from marine sponge producing salt tolerant proteases. pp. 31-39. ISSN 2180-1983

Protease is an important industrial enzyme and salt tolerant protease in which hasdesirableproperties that could enhance its uses not only in industries but also in agriculture and environmental. Marine organisms usually harbour halophilic microorganisms which produce salt tolerant protease. In this study salt tolerant protease producing bacteria from marine sponge were isolated and screened on skim milk marine agar supplemented with different NaCl concentrations (1.5 w/v). Out of 11 isolates 8 isolates (S1-1 S1-2 S1-3 S2-1 S2-3 S2-4 S2-5 and S2-6) showed clearing zones with ability to digest casein on the skim milk agar.Morphologically these strains are gram negative bacilli which grow in yellow colonies and were found to be catalase-positive but oxidase-negative. They are also non-lactose fermenter that produce gelatinase but not -amylase. The ribosomal 16S rRNA sequencing was used to identify each isolate (Acc. number of S1-1 for MT645770 S1-2 for MT645 771 S1-3 for MT645 772 S2-1 MT645 773 S2-3 MT645 774 S2-4 MT645 775 S2-5 MT645 776 and S2-6 MT645 7767). The 16S rRNA sequences showed that these isolates were highly similar to Stenotrophomonas maltophilia(S2-6 99.87) and to a related strain Pseudomonas hibiscicola(S1-1 S1-2 S1-3 S2-1 S2-3 S2-4 S2-5 (97.16-99.9 ). Further proteolytic studies were carried out using skim milk agar with 1.0 0.02 3.0 and 4.o (w/v) of NaCl concentrations. All isolates were able to hydrolyze case in which produced clear zones surrounding each colony at 1.0 and 2.0 (w/v) salt. However only isolate S1-5 and S2-6 showed proteolytic activities at 3.0 (w/v) salt but none of them at 4.0 (w/v). The ability of these isolates to produce protease which active at higher salt may indicate their potential to be the sources for enzyme with useful properties.

Full text available from: Official URL: https://journal.unisza.edu.my/agrobiotechnology/in...

Abstract

Protease is an important industrial enzyme and salt tolerant protease in which hasdesirableproperties that could enhance its uses not only in industries but also in agriculture and environmental. Marine organisms usually harbour halophilic microorganisms which produce salt tolerant protease. In this study salt tolerant protease producing bacteria from marine sponge were isolated and screened on skim milk marine agar supplemented with different NaCl concentrations (1.5 w/v). Out of 11 isolates 8 isolates (S1-1 S1-2 S1-3 S2-1 S2-3 S2-4 S2-5 and S2-6) showed clearing zones with ability to digest casein on the skim milk agar.Morphologically these strains are gram negative bacilli which grow in yellow colonies and were found to be catalase-positive but oxidase-negative. They are also non-lactose fermenter that produce gelatinase but not -amylase. The ribosomal 16S rRNA sequencing was used to identify each isolate (Acc. number of S1-1 for MT645770 S1-2 for MT645 771 S1-3 for MT645 772 S2-1 MT645 773 S2-3 MT645 774 S2-4 MT645 775 S2-5 MT645 776 and S2-6 MT645 7767). The 16S rRNA sequences showed that these isolates were highly similar to Stenotrophomonas maltophilia(S2-6 99.87) and to a related strain Pseudomonas hibiscicola(S1-1 S1-2 S1-3 S2-1 S2-3 S2-4 S2-5 (97.16-99.9 ). Further proteolytic studies were carried out using skim milk agar with 1.0 0.02 3.0 and 4.o (w/v) of NaCl concentrations. All isolates were able to hydrolyze case in which produced clear zones surrounding each colony at 1.0 and 2.0 (w/v) salt. However only isolate S1-5 and S2-6 showed proteolytic activities at 3.0 (w/v) salt but none of them at 4.0 (w/v). The ability of these isolates to produce protease which active at higher salt may indicate their potential to be the sources for enzyme with useful properties.

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Item Type:	Article
AGROVOC Term:	Proteases
AGROVOC Term:	Salt tolerance
AGROVOC Term:	Sampling
AGROVOC Term:	Agriculture
AGROVOC Term:	Environmental biology
AGROVOC Term:	Bioremediation
AGROVOC Term:	Biotechnological safety
Depositing User:	Mr. AFANDI ABDUL MALEK
Last Modified:	24 Apr 2025 00:55
URI:	http://webagris.upm.edu.my/id/eprint/9933

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