Expression of cadherin gene in Bacillus thuringiensis Cry1A toxin resistant and susceptible strains of Tirathaba mundella

Su Chong Ming, . and King Patricia Jie Hung, . and Ho Kok Lian, . and Lau Wei Hong, . and Tan Calvin Zhe Khai, . Expression of cadherin gene in Bacillus thuringiensis Cry1A toxin resistant and susceptible strains of Tirathaba mundella. pp. 248-256. ISSN 1511-2780

Bacillus thuringiensis (Bt.) insecticide has since been used as an essential insecticide in commercial oil palm plantations to manage insect pests. Resistance against Bt. toxins has been reported in many pest insects and threatening the effectiveness of Bt. insecticide. However the insecticide resistance capability of Tirathaba mundella (T. mundella) was not well documented due to its finite molecular data. In this study the potential of insecticide resistance development in T. mundella was examined through bioassay and cadherin gene expression analysis. Cadherin gene which often associated with the resistance against Bt. toxin was amplified and its relative expressions were studied using quantitative polymerase chain reactions. T. mundella collected from the plantations with frequent exposure to Bt. insecticide was found to develop resistance to Cry1A with a significantly lower cadherin gene transcript level and lower mortality compared to the susceptible strain. Besides a relatively lower level of cadherin gene expression was found in the early development stages of the resistant strain. The adaptation of larvae to the Bt. insecticide may be caused by the irregular application of insecticide in the field.

Full text available from: Official URL: http://jopr.mpob.gov.my/wp-content/uploads/2021/06...

Abstract

Bacillus thuringiensis (Bt.) insecticide has since been used as an essential insecticide in commercial oil palm plantations to manage insect pests. Resistance against Bt. toxins has been reported in many pest insects and threatening the effectiveness of Bt. insecticide. However the insecticide resistance capability of Tirathaba mundella (T. mundella) was not well documented due to its finite molecular data. In this study the potential of insecticide resistance development in T. mundella was examined through bioassay and cadherin gene expression analysis. Cadherin gene which often associated with the resistance against Bt. toxin was amplified and its relative expressions were studied using quantitative polymerase chain reactions. T. mundella collected from the plantations with frequent exposure to Bt. insecticide was found to develop resistance to Cry1A with a significantly lower cadherin gene transcript level and lower mortality compared to the susceptible strain. Besides a relatively lower level of cadherin gene expression was found in the early development stages of the resistant strain. The adaptation of larvae to the Bt. insecticide may be caused by the irregular application of insecticide in the field.

[error in script]

Item Type:	Article
AGROVOC Term:	Bacillus thuringiensis
AGROVOC Term:	Plant insecticides
AGROVOC Term:	Oil palm
AGROVOC Term:	Plantations
AGROVOC Term:	Insect pests
AGROVOC Term:	Resistance to insecticides
AGROVOC Term:	Polymerase chain reaction
AGROVOC Term:	Pest control
AGROVOC Term:	Pest control methods
Depositing User:	Mr. AFANDI ABDUL MALEK
Last Modified:	24 Apr 2025 00:55
URI:	http://webagris.upm.edu.my/id/eprint/9951

Actions (login required)

View Item