Isolation and characterisation of gibberellin 2-oxidase 6 gene from oil palm (Elaeis guineensis Jacq.)

Nurniwalis A. W., . and Zatty Syamimi Adura M. S., . and Zubaidah R., . Isolation and characterisation of gibberellin 2-oxidase 6 gene from oil palm (Elaeis guineensis Jacq.). pp. 235-247. ISSN 1511-2780

To increase our understanding on the role of Gibberellin (GA) metabolism in oil palm (Elaeis guineensis) a gene encoding enzyme in GA catabolic pathways was identified and isolated. The oligonucleotide primers were constructed and used to amplify the EgGA2ox6 gene from oil palm. The sequence analysis of EgGA2ox6 gene provided a complete open reading frame (ORF) of 1014 bp which encodes 337 deduced amino acid residues. Homology sequence analysis has shown a 50-70 sequence similarity to GA from other plant species. Based on this sequence motif analysis the EgGA2ox6 is classified into C‚�‚-GA2ox group. Expression studies on 13 different oil palm tissues showed that the highest expression profile of the gene was observed in the mesocarp tissue. Further expression analysis was conducted on the development stages of mesocarp using quantitative real-time polymerase chain reaction (qRT-PCR) showed that the gene was predominantly expressed in the early stages of mesocarp development at four weeks after anthesis (WAA). Then the expression of EgGA2ox6 gene was gradually reduced as the fruit ripened. This preliminary study provides valuable resources to further elucidate the roles of GA2ox6 gene during oil palm fruit development.

Full text available from: Official URL: http://jopr.mpob.gov.my/wp-content/uploads/2021/06...

Abstract

To increase our understanding on the role of Gibberellin (GA) metabolism in oil palm (Elaeis guineensis) a gene encoding enzyme in GA catabolic pathways was identified and isolated. The oligonucleotide primers were constructed and used to amplify the EgGA2ox6 gene from oil palm. The sequence analysis of EgGA2ox6 gene provided a complete open reading frame (ORF) of 1014 bp which encodes 337 deduced amino acid residues. Homology sequence analysis has shown a 50-70 sequence similarity to GA from other plant species. Based on this sequence motif analysis the EgGA2ox6 is classified into C‚�‚-GA2ox group. Expression studies on 13 different oil palm tissues showed that the highest expression profile of the gene was observed in the mesocarp tissue. Further expression analysis was conducted on the development stages of mesocarp using quantitative real-time polymerase chain reaction (qRT-PCR) showed that the gene was predominantly expressed in the early stages of mesocarp development at four weeks after anthesis (WAA). Then the expression of EgGA2ox6 gene was gradually reduced as the fruit ripened. This preliminary study provides valuable resources to further elucidate the roles of GA2ox6 gene during oil palm fruit development.

[error in script]

Item Type:	Article
AGROVOC Term:	Oil palm
AGROVOC Term:	Elaeis guineensis
AGROVOC Term:	Genes
AGROVOC Term:	Gibberellins
AGROVOC Term:	Metabolism
AGROVOC Term:	Isolation
AGROVOC Term:	Plant tissues
AGROVOC Term:	Mesocarp
AGROVOC Term:	Early development
AGROVOC Term:	Plant developmental stages
Depositing User:	Mr. AFANDI ABDUL MALEK
Last Modified:	24 Apr 2025 00:55
URI:	http://webagris.upm.edu.my/id/eprint/9952

Actions (login required)

View Item