Citation
Kadir M. A., . and Aziz M. A., . and Suhaimi N., . and Marziah M., . and Kabir M. H., . (2000) Introduction of green-fluorescent protein GFP gene in Musa acuminata cv. mas AA. [Proceedings Paper]
Abstract
In order to introduce genes with agronomic value into banana genetic transformation protocols have to be optimized. A PDS-1000/Helium delivery system was used to introduce the plasmid vector pGEM which carries the GFP gene driven by the Ubi 1 promoter into the scalp and embryogenic cell suspension of banana cv. Mas. Three 3 bombardment variables that affect transformation efficiency were examined which were the gold microcarrier size of 1.0 I-lm rupture disk pressure of 900 1100 and 1350 psi and the target distance of 6 9 and 12 cm. Transient GFP gene expression was assayed I to 14 days postbombardment which revealed that the highest number of transient transformants of scalp was obtained with the helium pressure of 1100 and 1350 psi along with a target distance of 9 cm and the highest number of transient transformants of embryogenic cell suspension was attained at 900 psi helium pressure with a target distance of 6 cm.
Download File
Full text available from:
|
Abstract
In order to introduce genes with agronomic value into banana genetic transformation protocols have to be optimized. A PDS-1000/Helium delivery system was used to introduce the plasmid vector pGEM which carries the GFP gene driven by the Ubi 1 promoter into the scalp and embryogenic cell suspension of banana cv. Mas. Three 3 bombardment variables that affect transformation efficiency were examined which were the gold microcarrier size of 1.0 I-lm rupture disk pressure of 900 1100 and 1350 psi and the target distance of 6 9 and 12 cm. Transient GFP gene expression was assayed I to 14 days postbombardment which revealed that the highest number of transient transformants of scalp was obtained with the helium pressure of 1100 and 1350 psi along with a target distance of 9 cm and the highest number of transient transformants of embryogenic cell suspension was attained at 900 psi helium pressure with a target distance of 6 cm.
Additional Metadata
Item Type: | Proceedings Paper |
---|---|
Additional Information: | Available at Perpustakaan Sultan Abdul Samad Universiti Putra Malaysia 43400 UPM Serdang Selangor Malaysia. QK710.2 M239 2000 Call Number |
AGROVOC Term: | Musa bananas |
AGROVOC Term: | Musa acuminata |
AGROVOC Term: | Musaceae |
AGROVOC Term: | Cultivars |
AGROVOC Term: | Genetic transformation |
AGROVOC Term: | Plasmids |
AGROVOC Term: | Gene vectors |
AGROVOC Term: | Suspension culture |
AGROVOC Term: | Proteins |
AGROVOC Term: | Gene expression |
Geographical Term: | MALAYSIA |
Depositing User: | Ms. Suzila Mohamad Kasim |
Last Modified: | 24 Apr 2025 05:15 |
URI: | http://webagris.upm.edu.my/id/eprint/12942 |
Actions (login required)
![]() |
View Item |