Introduction of green-fluorescent protein GFP gene in Musa acuminata cv. mas AA.

Kadir M. A., . and Aziz M. A., . and Suhaimi N., . and Marziah M., . and Kabir M. H., . (2000) Introduction of green-fluorescent protein GFP gene in Musa acuminata cv. mas AA. [Proceedings Paper]

In order to introduce genes with agronomic value into banana genetic transformation protocols have to be optimized. A PDS-1000/Helium delivery system was used to introduce the plasmid vector pGEM which carries the GFP gene driven by the Ubi 1 promoter into the scalp and embryogenic cell suspension of banana cv. Mas. Three 3 bombardment variables that affect transformation efficiency were examined which were the gold microcarrier size of 1.0 I-lm rupture disk pressure of 900 1100 and 1350 psi and the target distance of 6 9 and 12 cm. Transient GFP gene expression was assayed I to 14 days postbombardment which revealed that the highest number of transient transformants of scalp was obtained with the helium pressure of 1100 and 1350 psi along with a target distance of 9 cm and the highest number of transient transformants of embryogenic cell suspension was attained at 900 psi helium pressure with a target distance of 6 cm.

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Abstract

In order to introduce genes with agronomic value into banana genetic transformation protocols have to be optimized. A PDS-1000/Helium delivery system was used to introduce the plasmid vector pGEM which carries the GFP gene driven by the Ubi 1 promoter into the scalp and embryogenic cell suspension of banana cv. Mas. Three 3 bombardment variables that affect transformation efficiency were examined which were the gold microcarrier size of 1.0 I-lm rupture disk pressure of 900 1100 and 1350 psi and the target distance of 6 9 and 12 cm. Transient GFP gene expression was assayed I to 14 days postbombardment which revealed that the highest number of transient transformants of scalp was obtained with the helium pressure of 1100 and 1350 psi along with a target distance of 9 cm and the highest number of transient transformants of embryogenic cell suspension was attained at 900 psi helium pressure with a target distance of 6 cm.

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Item Type:	Proceedings Paper
Additional Information:	Available at Perpustakaan Sultan Abdul Samad Universiti Putra Malaysia 43400 UPM Serdang Selangor Malaysia. QK710.2 M239 2000 Call Number
AGROVOC Term:	Musa bananas
AGROVOC Term:	Musa acuminata
AGROVOC Term:	Musaceae
AGROVOC Term:	Cultivars
AGROVOC Term:	Genetic transformation
AGROVOC Term:	Plasmids
AGROVOC Term:	Gene vectors
AGROVOC Term:	Suspension culture
AGROVOC Term:	Proteins
AGROVOC Term:	Gene expression
Geographical Term:	MALAYSIA
Depositing User:	Ms. Suzila Mohamad Kasim
Last Modified:	24 Apr 2025 05:15
URI:	http://webagris.upm.edu.my/id/eprint/12942

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