Citation
Hari Kumar Krishnan, . and Kalaivani Nadarajah, . and Amir Rabu, . and Wickneswari V. Ratnam, . (2011) Isolation and characterisation of proteins glycoproteins and volatiles induced by Magnaporthe grisea elicitors. [Proceedings Paper]
Abstract
Rice blast disease caused by the fungus Magnaporthe grisea is one of the most devastating diseases of cultivated rice throughout the world. Plants are equipped with variety of mechanisms that enable them to defend themselves against pathogens. These defense responses include the hypersensitive response phytoalexin accumulation oxidative burst and an increased expression of defense-related genes The formation of these various chemical and physical barriers is induced by molecules called elicitors that are produced by the pathogens. In this research we have targeted the protein and glycoprotein elicitors derived from the pathogen itself Using MALDI-TOF and LC-MS analysis we have found the components of elicitor to be similar to lectin Concanavalin A and also a putative uncharacterized protein of M.grisea. Rice leaves were treated with elicitors and the volatiles were collected using solid-phase microextraction technique and analyzed using GC-MS. We identified monoterpenes and sesquiterpenes as volatiles induced by these elicitors in line with a previously done research using a different rice species and cultivar.
Download File
Full text available from:
|
Abstract
Rice blast disease caused by the fungus Magnaporthe grisea is one of the most devastating diseases of cultivated rice throughout the world. Plants are equipped with variety of mechanisms that enable them to defend themselves against pathogens. These defense responses include the hypersensitive response phytoalexin accumulation oxidative burst and an increased expression of defense-related genes The formation of these various chemical and physical barriers is induced by molecules called elicitors that are produced by the pathogens. In this research we have targeted the protein and glycoprotein elicitors derived from the pathogen itself Using MALDI-TOF and LC-MS analysis we have found the components of elicitor to be similar to lectin Concanavalin A and also a putative uncharacterized protein of M.grisea. Rice leaves were treated with elicitors and the volatiles were collected using solid-phase microextraction technique and analyzed using GC-MS. We identified monoterpenes and sesquiterpenes as volatiles induced by these elicitors in line with a previously done research using a different rice species and cultivar.
Additional Metadata
Item Type: | Proceedings Paper |
---|---|
Additional Information: | Available at Perpustakaan Sultan Abdul Samad Universiti Putra Malaysia 43400 UPM Serdang Selangor Malaysia. QR22 M3I61 2011 vol.2 Call Number |
AGROVOC Term: | Magnaporthe grisea |
AGROVOC Term: | Oryza |
AGROVOC Term: | Oryza sativa |
AGROVOC Term: | Plant diseases |
AGROVOC Term: | Isolation |
AGROVOC Term: | Purification |
AGROVOC Term: | Leaves |
AGROVOC Term: | Acrylamide |
AGROVOC Term: | Gas chromatography |
AGROVOC Term: | Mass spectrometry |
Geographical Term: | MALAYSIA |
Depositing User: | Ms. Suzila Mohamad Kasim |
Last Modified: | 24 Apr 2025 05:15 |
URI: | http://webagris.upm.edu.my/id/eprint/12991 |
Actions (login required)
![]() |
View Item |