Isolation and characterisation of proteins glycoproteins and volatiles induced by Magnaporthe grisea elicitors

Hari Kumar Krishnan, . and Kalaivani Nadarajah, . and Amir Rabu, . and Wickneswari V. Ratnam, . (2011) Isolation and characterisation of proteins glycoproteins and volatiles induced by Magnaporthe grisea elicitors. [Proceedings Paper]

Rice blast disease caused by the fungus Magnaporthe grisea is one of the most devastating diseases of cultivated rice throughout the world. Plants are equipped with variety of mechanisms that enable them to defend themselves against pathogens. These defense responses include the hypersensitive response phytoalexin accumulation oxidative burst and an increased expression of defense-related genes The formation of these various chemical and physical barriers is induced by molecules called elicitors that are produced by the pathogens. In this research we have targeted the protein and glycoprotein elicitors derived from the pathogen itself Using MALDI-TOF and LC-MS analysis we have found the components of elicitor to be similar to lectin Concanavalin A and also a putative uncharacterized protein of M.grisea. Rice leaves were treated with elicitors and the volatiles were collected using solid-phase microextraction technique and analyzed using GC-MS. We identified monoterpenes and sesquiterpenes as volatiles induced by these elicitors in line with a previously done research using a different rice species and cultivar.

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Abstract

Rice blast disease caused by the fungus Magnaporthe grisea is one of the most devastating diseases of cultivated rice throughout the world. Plants are equipped with variety of mechanisms that enable them to defend themselves against pathogens. These defense responses include the hypersensitive response phytoalexin accumulation oxidative burst and an increased expression of defense-related genes The formation of these various chemical and physical barriers is induced by molecules called elicitors that are produced by the pathogens. In this research we have targeted the protein and glycoprotein elicitors derived from the pathogen itself Using MALDI-TOF and LC-MS analysis we have found the components of elicitor to be similar to lectin Concanavalin A and also a putative uncharacterized protein of M.grisea. Rice leaves were treated with elicitors and the volatiles were collected using solid-phase microextraction technique and analyzed using GC-MS. We identified monoterpenes and sesquiterpenes as volatiles induced by these elicitors in line with a previously done research using a different rice species and cultivar.

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Item Type:	Proceedings Paper
Additional Information:	Available at Perpustakaan Sultan Abdul Samad Universiti Putra Malaysia 43400 UPM Serdang Selangor Malaysia. QR22 M3I61 2011 vol.2 Call Number
AGROVOC Term:	Magnaporthe grisea
AGROVOC Term:	Oryza
AGROVOC Term:	Oryza sativa
AGROVOC Term:	Plant diseases
AGROVOC Term:	Isolation
AGROVOC Term:	Purification
AGROVOC Term:	Leaves
AGROVOC Term:	Acrylamide
AGROVOC Term:	Gas chromatography
AGROVOC Term:	Mass spectrometry
Geographical Term:	MALAYSIA
Depositing User:	Ms. Suzila Mohamad Kasim
Last Modified:	24 Apr 2025 05:15
URI:	http://webagris.upm.edu.my/id/eprint/12991

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