Citation
Fatimah Abdullah, . and Amir Rabu, . and Raha Abdul Rahim, . and Rosli Md. lIIias, . (2011) Proteomics analysis of the effect of growth media on folate biosynthesis by local isolated Lactococcus lactis strains M4 and M12. [Proceedings Paper]
Abstract
Lactococcus lactis is by far the most extensively studied lactic acid bacterium and an ideal model organism for metabolic engineering strategies. The dairy starter bacterium L. lactis has the potential to synthesise both folate vitamin B11 and riboflavin vitamin B2. L. lactis strains M4 and M12 are lactic acid bacteria that have been locally isolated from Malaysia. In order to understand the regulation and protein expression in these bacteria under certain conditions the proteome analysis of L. lactis for these strains was performed. Folate concentration was determined by using the microbiological assay while the protein profile was determined by using liquid chromatography mass spectrometry LCMS. The expression of folate proteins expressed by the L. lactis strains in two different conditions are described in this study.
Download File
Full text available from:
|
Abstract
Lactococcus lactis is by far the most extensively studied lactic acid bacterium and an ideal model organism for metabolic engineering strategies. The dairy starter bacterium L. lactis has the potential to synthesise both folate vitamin B11 and riboflavin vitamin B2. L. lactis strains M4 and M12 are lactic acid bacteria that have been locally isolated from Malaysia. In order to understand the regulation and protein expression in these bacteria under certain conditions the proteome analysis of L. lactis for these strains was performed. Folate concentration was determined by using the microbiological assay while the protein profile was determined by using liquid chromatography mass spectrometry LCMS. The expression of folate proteins expressed by the L. lactis strains in two different conditions are described in this study.
Additional Metadata
| Item Type: | Proceedings Paper |
|---|---|
| Additional Information: | Available at Perpustakaan Sultan Abdul Samad Universiti Putra Malaysia 43400 UPM Serdang Selangor Malaysia. QR22 M3I61 2011 vol.2 Call Number. |
| AGROVOC Term: | Lactococcus lactis |
| AGROVOC Term: | Lactic acid bacteria |
| AGROVOC Term: | Microbiological analysis |
| AGROVOC Term: | Isolation of microorganisms |
| AGROVOC Term: | Biosynthesis |
| AGROVOC Term: | Gas liquid chromatography |
| AGROVOC Term: | Mass spectrometry |
| AGROVOC Term: | Vitamin B1 |
| AGROVOC Term: | Vitamin B2 |
| AGROVOC Term: | Riboflavin |
| Geographical Term: | MALAYSIA |
| Depositing User: | Ms. Suzila Mohamad Kasim |
| Last Modified: | 24 Apr 2025 05:15 |
| URI: | http://webagris.upm.edu.my/id/eprint/13066 |
Actions (login required)
 |
View Item |
