Proteomics analysis of the effect of growth media on folate biosynthesis by local isolated Lactococcus lactis strains M4 and M12


Citation

Fatimah Abdullah, . and Amir Rabu, . and Raha Abdul Rahim, . and Rosli Md. lIIias, . (2011) Proteomics analysis of the effect of growth media on folate biosynthesis by local isolated Lactococcus lactis strains M4 and M12. [Proceedings Paper]

Abstract

Lactococcus lactis is by far the most extensively studied lactic acid bacterium and an ideal model organism for metabolic engineering strategies. The dairy starter bacterium L. lactis has the potential to synthesise both folate vitamin B11 and riboflavin vitamin B2. L. lactis strains M4 and M12 are lactic acid bacteria that have been locally isolated from Malaysia. In order to understand the regulation and protein expression in these bacteria under certain conditions the proteome analysis of L. lactis for these strains was performed. Folate concentration was determined by using the microbiological assay while the protein profile was determined by using liquid chromatography mass spectrometry LCMS. The expression of folate proteins expressed by the L. lactis strains in two different conditions are described in this study.


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Abstract

Lactococcus lactis is by far the most extensively studied lactic acid bacterium and an ideal model organism for metabolic engineering strategies. The dairy starter bacterium L. lactis has the potential to synthesise both folate vitamin B11 and riboflavin vitamin B2. L. lactis strains M4 and M12 are lactic acid bacteria that have been locally isolated from Malaysia. In order to understand the regulation and protein expression in these bacteria under certain conditions the proteome analysis of L. lactis for these strains was performed. Folate concentration was determined by using the microbiological assay while the protein profile was determined by using liquid chromatography mass spectrometry LCMS. The expression of folate proteins expressed by the L. lactis strains in two different conditions are described in this study.

Additional Metadata

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Item Type: Proceedings Paper
Additional Information: Available at Perpustakaan Sultan Abdul Samad Universiti Putra Malaysia 43400 UPM Serdang Selangor Malaysia. QR22 M3I61 2011 vol.2 Call Number.
AGROVOC Term: Lactococcus lactis
AGROVOC Term: Lactic acid bacteria
AGROVOC Term: Microbiological analysis
AGROVOC Term: Isolation of microorganisms
AGROVOC Term: Biosynthesis
AGROVOC Term: Gas liquid chromatography
AGROVOC Term: Mass spectrometry
AGROVOC Term: Vitamin B1
AGROVOC Term: Vitamin B2
AGROVOC Term: Riboflavin
Geographical Term: MALAYSIA
Depositing User: Ms. Suzila Mohamad Kasim
Last Modified: 24 Apr 2025 05:15
URI: http://webagris.upm.edu.my/id/eprint/13066

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