Citation
Idris A. S., . and Yuvarani N., . and Shamala S., . (2011) In vitro screening of potential basidiomycetes fungi for biocontrol and biodegradation characteristics against Ganoderma boninense the causal agent of basal stem rot of oil palm. [Proceedings Paper]
Abstract
A total of 25 Basidiomycetes fungi were isolated from within various fungal fruiting bodies thatwere collected from dead oil palm trunk. All the isolates were tested in dual culture assayagainst Ganoderma boninense on PDA plates and in vitro degradation of lignin cellulose starch and xylan respectively. Results indicated that percentage inhibition of radial growth PIRG in dual culture was higher than 80 against G. boninense and were recorded by the five isolates namely FBR FBB FBB UKM2 FBW and ST3. Isolates of ST2 PTG 13 STB PTG 12 and FBJ G3 recorded highly significant P 0.05 values of dark brown zone mm of 71.72 70.9 68 64.3 and 63.2 respectivley indicating the ability of these isolates in degrading lignin through the release of polyphenol oxidase on media containing tannic acid. Isolates of PTG 13 and PTG 12 produced significantly higher P 0.05 clearing zone values of 84.5 mm and 84.35 mm respectively on carboxymethyl cellulose amended media via the synthesis of endoglucanase. Isolates of PTG 12 PTG 13 STB and FBW exhibited the highest ability to degrade starch with strong degradation reaction which indicates the presence of amylase enzymes. Additionally PTG 12 PTG 13 and FBW were also proven to effectively degrade xylanwith strong degradation reaction followed by FBJ G3 ST2 ST3 FBB and FBB UKM2. All potential isolates will be subjected to further screening for the in vivo studies.
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Abstract
A total of 25 Basidiomycetes fungi were isolated from within various fungal fruiting bodies thatwere collected from dead oil palm trunk. All the isolates were tested in dual culture assayagainst Ganoderma boninense on PDA plates and in vitro degradation of lignin cellulose starch and xylan respectively. Results indicated that percentage inhibition of radial growth PIRG in dual culture was higher than 80 against G. boninense and were recorded by the five isolates namely FBR FBB FBB UKM2 FBW and ST3. Isolates of ST2 PTG 13 STB PTG 12 and FBJ G3 recorded highly significant P 0.05 values of dark brown zone mm of 71.72 70.9 68 64.3 and 63.2 respectivley indicating the ability of these isolates in degrading lignin through the release of polyphenol oxidase on media containing tannic acid. Isolates of PTG 13 and PTG 12 produced significantly higher P 0.05 clearing zone values of 84.5 mm and 84.35 mm respectively on carboxymethyl cellulose amended media via the synthesis of endoglucanase. Isolates of PTG 12 PTG 13 STB and FBW exhibited the highest ability to degrade starch with strong degradation reaction which indicates the presence of amylase enzymes. Additionally PTG 12 PTG 13 and FBW were also proven to effectively degrade xylanwith strong degradation reaction followed by FBJ G3 ST2 ST3 FBB and FBB UKM2. All potential isolates will be subjected to further screening for the in vivo studies.
Additional Metadata
Item Type: | Proceedings Paper |
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Additional Information: | Available at Perpustakaan Sultan Abdul Samad Universiti Putra Malaysia 43400 UPM Serdang Selangor Malaysia. SB608 O27M939 2011 Call Number. |
AGROVOC Term: | Oil palm |
AGROVOC Term: | Plant disease control |
AGROVOC Term: | Ganoderma |
AGROVOC Term: | Screening tests |
AGROVOC Term: | Biological techniques in vitro |
AGROVOC Term: | Basidiomycetes |
AGROVOC Term: | Fungi |
AGROVOC Term: | Biocontrol |
AGROVOC Term: | Biodegradation |
AGROVOC Term: | Biological control agents |
Geographical Term: | MALAYSIA |
Depositing User: | Ms. Suzila Mohamad Kasim |
Last Modified: | 24 Apr 2025 05:16 |
URI: | http://webagris.upm.edu.my/id/eprint/13677 |
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