In vitro screening of potential basidiomycetes fungi for biocontrol and biodegradation characteristics against Ganoderma boninense the causal agent of basal stem rot of oil palm

Idris A. S., . and Yuvarani N., . and Shamala S., . (2011) In vitro screening of potential basidiomycetes fungi for biocontrol and biodegradation characteristics against Ganoderma boninense the causal agent of basal stem rot of oil palm. [Proceedings Paper]

A total of 25 Basidiomycetes fungi were isolated from within various fungal fruiting bodies thatwere collected from dead oil palm trunk. All the isolates were tested in dual culture assayagainst Ganoderma boninense on PDA plates and in vitro degradation of lignin cellulose starch and xylan respectively. Results indicated that percentage inhibition of radial growth PIRG in dual culture was higher than 80 against G. boninense and were recorded by the five isolates namely FBR FBB FBB UKM2 FBW and ST3. Isolates of ST2 PTG 13 STB PTG 12 and FBJ G3 recorded highly significant P 0.05 values of dark brown zone mm of 71.72 70.9 68 64.3 and 63.2 respectivley indicating the ability of these isolates in degrading lignin through the release of polyphenol oxidase on media containing tannic acid. Isolates of PTG 13 and PTG 12 produced significantly higher P 0.05 clearing zone values of 84.5 mm and 84.35 mm respectively on carboxymethyl cellulose amended media via the synthesis of endoglucanase. Isolates of PTG 12 PTG 13 STB and FBW exhibited the highest ability to degrade starch with strong degradation reaction which indicates the presence of amylase enzymes. Additionally PTG 12 PTG 13 and FBW were also proven to effectively degrade xylanwith strong degradation reaction followed by FBJ G3 ST2 ST3 FBB and FBB UKM2. All potential isolates will be subjected to further screening for the in vivo studies.

Full text available from:

Abstract

A total of 25 Basidiomycetes fungi were isolated from within various fungal fruiting bodies thatwere collected from dead oil palm trunk. All the isolates were tested in dual culture assayagainst Ganoderma boninense on PDA plates and in vitro degradation of lignin cellulose starch and xylan respectively. Results indicated that percentage inhibition of radial growth PIRG in dual culture was higher than 80 against G. boninense and were recorded by the five isolates namely FBR FBB FBB UKM2 FBW and ST3. Isolates of ST2 PTG 13 STB PTG 12 and FBJ G3 recorded highly significant P 0.05 values of dark brown zone mm of 71.72 70.9 68 64.3 and 63.2 respectivley indicating the ability of these isolates in degrading lignin through the release of polyphenol oxidase on media containing tannic acid. Isolates of PTG 13 and PTG 12 produced significantly higher P 0.05 clearing zone values of 84.5 mm and 84.35 mm respectively on carboxymethyl cellulose amended media via the synthesis of endoglucanase. Isolates of PTG 12 PTG 13 STB and FBW exhibited the highest ability to degrade starch with strong degradation reaction which indicates the presence of amylase enzymes. Additionally PTG 12 PTG 13 and FBW were also proven to effectively degrade xylanwith strong degradation reaction followed by FBJ G3 ST2 ST3 FBB and FBB UKM2. All potential isolates will be subjected to further screening for the in vivo studies.

[error in script]

Item Type:	Proceedings Paper
Additional Information:	Available at Perpustakaan Sultan Abdul Samad Universiti Putra Malaysia 43400 UPM Serdang Selangor Malaysia. SB608 O27M939 2011 Call Number.
AGROVOC Term:	Oil palm
AGROVOC Term:	Plant disease control
AGROVOC Term:	Ganoderma
AGROVOC Term:	Screening tests
AGROVOC Term:	Biological techniques in vitro
AGROVOC Term:	Basidiomycetes
AGROVOC Term:	Fungi
AGROVOC Term:	Biocontrol
AGROVOC Term:	Biodegradation
AGROVOC Term:	Biological control agents
Geographical Term:	MALAYSIA
Depositing User:	Ms. Suzila Mohamad Kasim
Last Modified:	24 Apr 2025 05:16
URI:	http://webagris.upm.edu.my/id/eprint/13677

Actions (login required)

View Item