The potential of gene cloning in thermophilic bacteria


Citation

Koh C.L., . (1984) The potential of gene cloning in thermophilic bacteria. [Proceedings Paper]

Abstract

A covalently closed circular supercoiled plasmid pCLK 1 was detected in the caldoactive thermophilic bacterium Thermus icelandic strain B1. It was subsequently purified by caesium chlorideethidium bromide density gradient centrifugation. A physical map of pCLK 1 showing the cleavage sites of nine restriction endonucleases was constructed. Its size was determined to be 8.76 kb. The implications of this finding are discussed


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Abstract

A covalently closed circular supercoiled plasmid pCLK 1 was detected in the caldoactive thermophilic bacterium Thermus icelandic strain B1. It was subsequently purified by caesium chlorideethidium bromide density gradient centrifugation. A physical map of pCLK 1 showing the cleavage sites of nine restriction endonucleases was constructed. Its size was determined to be 8.76 kb. The implications of this finding are discussed

Additional Metadata

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Item Type: Proceedings Paper
Additional Information: 5 ill.; 27 ref. Summary En
AGROVOC Term: BACTERIA
AGROVOC Term: GENES
AGROVOC Term: CLONES
Geographical Term: MALAYSIA
Depositing User: Ms. Norfaezah Khomsan
Last Modified: 24 Apr 2025 05:25
URI: http://webagris.upm.edu.my/id/eprint/14120

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