Regeneration and Agrobacterium-mediated transformation of cucumber Cucumis sativus and muskmelon Cucumis melo

Ramlan Saimat, . and Mohi Uddin A.K.M., . and Suhaimi Napis, . (1995) Regeneration and Agrobacterium-mediated transformation of cucumber Cucumis sativus and muskmelon Cucumis melo. [Proceedings Paper]

Regeneration and Agrobacterium-mediated of cucumber Cucumis sativus and muskmelon Cucumis melo L. two of the more important commercial crop from the Family Cucurbitaceae were studied. The use of silver nitrate and cobalt chloride inhibitors of ethylene action and biosynthesis respectively was investigated on cucumber cv. Spring Swallow SS and Tasty Green TG and muskmelon cv. Birdie for their ability to enhance in vitro shoot regeneration. Poor regeneration was obtained cotyledon and hypocotyl explants placed on a medium containing Murashige and Skoog MS medium and different concentrations BAP. In contrast the percentage of shoot regeneration as well as number of shoots per explants increased when silver nitrate and cobalt chloride were added to the medium. For the transformation studies explants were co-cultured with Agrobacterium tumefaciens strain LBA4404 separately carrying plasmids pBI121 and pKTY03 containing CaMV 35S promoter and PAL promoter respectively with kanamycin resistance and GUS gene. Putative transgenic cucumber plants regenerated directly from cells of the explants leaf disc petiole cotyledon and hypocotyl. Kanamycin-resistant plants regenerated 3 to 4 weeks after transferring onto selective medium

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Abstract

Regeneration and Agrobacterium-mediated of cucumber Cucumis sativus and muskmelon Cucumis melo L. two of the more important commercial crop from the Family Cucurbitaceae were studied. The use of silver nitrate and cobalt chloride inhibitors of ethylene action and biosynthesis respectively was investigated on cucumber cv. Spring Swallow SS and Tasty Green TG and muskmelon cv. Birdie for their ability to enhance in vitro shoot regeneration. Poor regeneration was obtained cotyledon and hypocotyl explants placed on a medium containing Murashige and Skoog MS medium and different concentrations BAP. In contrast the percentage of shoot regeneration as well as number of shoots per explants increased when silver nitrate and cobalt chloride were added to the medium. For the transformation studies explants were co-cultured with Agrobacterium tumefaciens strain LBA4404 separately carrying plasmids pBI121 and pKTY03 containing CaMV 35S promoter and PAL promoter respectively with kanamycin resistance and GUS gene. Putative transgenic cucumber plants regenerated directly from cells of the explants leaf disc petiole cotyledon and hypocotyl. Kanamycin-resistant plants regenerated 3 to 4 weeks after transferring onto selective medium

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Item Type:	Proceedings Paper
Additional Information:	Summary En
AGROVOC Term:	CUCUMIS SATIVUS
AGROVOC Term:	CUCUMIS MELO
AGROVOC Term:	CULTIVO IN VITRO
AGROVOC Term:	REGENERACION IN VITRO
AGROVOC Term:	TRANSFORMACION GENETICA
AGROVOC Term:	AGROBACTERIUM TUMEFACIENS
AGROVOC Term:	PEPINO
AGROVOC Term:	MELON
Geographical Term:	MALAYSIA
Depositing User:	Ms. Norfaezah Khomsan
Last Modified:	24 Apr 2025 05:26
URI:	http://webagris.upm.edu.my/id/eprint/15384

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