Citation
Ramlan Saimat, . and Mohi Uddin A.K.M., . and Suhaimi Napis, . (1995) Regeneration and Agrobacterium-mediated transformation of cucumber Cucumis sativus and muskmelon Cucumis melo. [Proceedings Paper]
Abstract
Regeneration and Agrobacterium-mediated of cucumber Cucumis sativus and muskmelon Cucumis melo L. two of the more important commercial crop from the Family Cucurbitaceae were studied. The use of silver nitrate and cobalt chloride inhibitors of ethylene action and biosynthesis respectively was investigated on cucumber cv. Spring Swallow SS and Tasty Green TG and muskmelon cv. Birdie for their ability to enhance in vitro shoot regeneration. Poor regeneration was obtained cotyledon and hypocotyl explants placed on a medium containing Murashige and Skoog MS medium and different concentrations BAP. In contrast the percentage of shoot regeneration as well as number of shoots per explants increased when silver nitrate and cobalt chloride were added to the medium. For the transformation studies explants were co-cultured with Agrobacterium tumefaciens strain LBA4404 separately carrying plasmids pBI121 and pKTY03 containing CaMV 35S promoter and PAL promoter respectively with kanamycin resistance and GUS gene. Putative transgenic cucumber plants regenerated directly from cells of the explants leaf disc petiole cotyledon and hypocotyl. Kanamycin-resistant plants regenerated 3 to 4 weeks after transferring onto selective medium
Download File
Full text available from:
|
Abstract
Regeneration and Agrobacterium-mediated of cucumber Cucumis sativus and muskmelon Cucumis melo L. two of the more important commercial crop from the Family Cucurbitaceae were studied. The use of silver nitrate and cobalt chloride inhibitors of ethylene action and biosynthesis respectively was investigated on cucumber cv. Spring Swallow SS and Tasty Green TG and muskmelon cv. Birdie for their ability to enhance in vitro shoot regeneration. Poor regeneration was obtained cotyledon and hypocotyl explants placed on a medium containing Murashige and Skoog MS medium and different concentrations BAP. In contrast the percentage of shoot regeneration as well as number of shoots per explants increased when silver nitrate and cobalt chloride were added to the medium. For the transformation studies explants were co-cultured with Agrobacterium tumefaciens strain LBA4404 separately carrying plasmids pBI121 and pKTY03 containing CaMV 35S promoter and PAL promoter respectively with kanamycin resistance and GUS gene. Putative transgenic cucumber plants regenerated directly from cells of the explants leaf disc petiole cotyledon and hypocotyl. Kanamycin-resistant plants regenerated 3 to 4 weeks after transferring onto selective medium
Additional Metadata
Item Type: | Proceedings Paper |
---|---|
Additional Information: | Summary En |
AGROVOC Term: | CUCUMIS SATIVUS |
AGROVOC Term: | CUCUMIS MELO |
AGROVOC Term: | CULTIVO IN VITRO |
AGROVOC Term: | REGENERACION IN VITRO |
AGROVOC Term: | TRANSFORMACION GENETICA |
AGROVOC Term: | AGROBACTERIUM TUMEFACIENS |
AGROVOC Term: | PEPINO |
AGROVOC Term: | MELON |
Geographical Term: | MALAYSIA |
Depositing User: | Ms. Norfaezah Khomsan |
Last Modified: | 24 Apr 2025 05:26 |
URI: | http://webagris.upm.edu.my/id/eprint/15384 |
Actions (login required)
![]() |
View Item |