Separation of Y-chromosome bearing bulls spermatozoa using an albumin gradient technique

Jasmi Y., . and Tan Y.J., . and Musaddin K., . and Somarny W.W.M.Z., . and Mat Tasol S., . and Johari J.A., . Separation of Y-chromosome bearing bulls spermatozoa using an albumin gradient technique. pp. 13-19. ISSN 1394-3227

This study was conducted to separate the X- and Y-chromosome bearing spermatozoa based on an albumin gradient technique. A modified but simple procedure was performed to minimize pressure on the spermatozoa. Two treatments consisting of two-phase albumin gradients which were 12.5 over 20 (Treatment 1) and 15 over 20 (Treatment 2) bovine serum albumin (BSA) were carried out to separate the X- and Y-chromosome bearing bulls spermatozoa at room temperature. Subsequent analysis by FISH using a single labeled TSPY probe (specific for detection of Y-chromosome) showed that the albumin gradient technique was not effective (P0.05) to separate the X- and Y- chromosome bearing spermatozoa. The percentage of Y-chromosome bearing spermatozoa recovered following separation by Treatment 2 was higher but not significant (P0.05) compared to Treatment 1.

Full text available from: Official URL: http://www.msap.my/mjas/13-19 20%28Somarny%29_edit...

Abstract

This study was conducted to separate the X- and Y-chromosome bearing spermatozoa based on an albumin gradient technique. A modified but simple procedure was performed to minimize pressure on the spermatozoa. Two treatments consisting of two-phase albumin gradients which were 12.5 over 20 (Treatment 1) and 15 over 20 (Treatment 2) bovine serum albumin (BSA) were carried out to separate the X- and Y-chromosome bearing bulls spermatozoa at room temperature. Subsequent analysis by FISH using a single labeled TSPY probe (specific for detection of Y-chromosome) showed that the albumin gradient technique was not effective (P0.05) to separate the X- and Y- chromosome bearing spermatozoa. The percentage of Y-chromosome bearing spermatozoa recovered following separation by Treatment 2 was higher but not significant (P0.05) compared to Treatment 1.

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Item Type:	Article
AGROVOC Term:	Bulls
AGROVOC Term:	Bovines
AGROVOC Term:	Chromosomes
AGROVOC Term:	Spermatozoa
AGROVOC Term:	Albumins
Depositing User:	Ms. Suzila Mohamad Kasim
Last Modified:	24 Apr 2025 06:27
URI:	http://webagris.upm.edu.my/id/eprint/21562

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