Separation of Y-chromosome bearing bulls spermatozoa using an albumin gradient technique


Citation

Jasmi Y., . and Tan Y.J., . and Musaddin K., . and Somarny W.W.M.Z., . and Mat Tasol S., . and Johari J.A., . Separation of Y-chromosome bearing bulls spermatozoa using an albumin gradient technique. pp. 13-19. ISSN 1394-3227

Abstract

This study was conducted to separate the X- and Y-chromosome bearing spermatozoa based on an albumin gradient technique. A modified but simple procedure was performed to minimize pressure on the spermatozoa. Two treatments consisting of two-phase albumin gradients which were 12.5 over 20 (Treatment 1) and 15 over 20 (Treatment 2) bovine serum albumin (BSA) were carried out to separate the X- and Y-chromosome bearing bulls spermatozoa at room temperature. Subsequent analysis by FISH using a single labeled TSPY probe (specific for detection of Y-chromosome) showed that the albumin gradient technique was not effective (P0.05) to separate the X- and Y- chromosome bearing spermatozoa. The percentage of Y-chromosome bearing spermatozoa recovered following separation by Treatment 2 was higher but not significant (P0.05) compared to Treatment 1.


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Abstract

This study was conducted to separate the X- and Y-chromosome bearing spermatozoa based on an albumin gradient technique. A modified but simple procedure was performed to minimize pressure on the spermatozoa. Two treatments consisting of two-phase albumin gradients which were 12.5 over 20 (Treatment 1) and 15 over 20 (Treatment 2) bovine serum albumin (BSA) were carried out to separate the X- and Y-chromosome bearing bulls spermatozoa at room temperature. Subsequent analysis by FISH using a single labeled TSPY probe (specific for detection of Y-chromosome) showed that the albumin gradient technique was not effective (P0.05) to separate the X- and Y- chromosome bearing spermatozoa. The percentage of Y-chromosome bearing spermatozoa recovered following separation by Treatment 2 was higher but not significant (P0.05) compared to Treatment 1.

Additional Metadata

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Item Type: Article
AGROVOC Term: Bulls
AGROVOC Term: Bovines
AGROVOC Term: Chromosomes
AGROVOC Term: Spermatozoa
AGROVOC Term: Albumins
Depositing User: Ms. Suzila Mohamad Kasim
Last Modified: 24 Apr 2025 06:27
URI: http://webagris.upm.edu.my/id/eprint/21562

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