Identification and characterisation of endo-1 4--glucanase family members in Hevea brasiliensis


Citation

Chung H. H., . and Chow K. S., . and Thompson C., . and Mollison E., . Identification and characterisation of endo-1 4--glucanase family members in Hevea brasiliensis. pp. 148-158. ISSN 1511-1768

Abstract

In order to study wood formation in Hevea brasiliensis endo-1 4--glucanase (EGase) was selected for molecular genetic analysis. EGase sequences were isolated from bark leaf and latex transcriptomes (clone RRIM 928). Altogether 22 sequences were obtained from the bark transcriptome while 14 and 8 sequences were obtained from leaf and latex transcriptomes respectively. Based on multiple sequence alignment only twelve sequences encoded full protein open reading frames while the others contained partial proteins. Translation of twelve EGase sequences (designated HbEg1-12) produced peptide sequences ranging from 494-622 amino acids. Based on protein motif conservation and phylogenetic analysis these EGases were separated into three classes A B and C. Expression of EGase was found to be generally low in latex bark and leaves.


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Abstract

In order to study wood formation in Hevea brasiliensis endo-1 4--glucanase (EGase) was selected for molecular genetic analysis. EGase sequences were isolated from bark leaf and latex transcriptomes (clone RRIM 928). Altogether 22 sequences were obtained from the bark transcriptome while 14 and 8 sequences were obtained from leaf and latex transcriptomes respectively. Based on multiple sequence alignment only twelve sequences encoded full protein open reading frames while the others contained partial proteins. Translation of twelve EGase sequences (designated HbEg1-12) produced peptide sequences ranging from 494-622 amino acids. Based on protein motif conservation and phylogenetic analysis these EGases were separated into three classes A B and C. Expression of EGase was found to be generally low in latex bark and leaves.

Additional Metadata

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Item Type: Article
AGROVOC Term: Identification
AGROVOC Term: Beta glucanase
AGROVOC Term: Hevea brasiliensis
AGROVOC Term: Rubber
AGROVOC Term: Wood formation
AGROVOC Term: Molecular genetics
AGROVOC Term: Clones
AGROVOC Term: Amino acids
AGROVOC Term: Latex
AGROVOC Term: Leaves
Geographical Term: Malaysia
Depositing User: Ms. Suzila Mohamad Kasim
Last Modified: 28 Apr 2025 00:41
URI: http://webagris.upm.edu.my/id/eprint/22941

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