Citation
Chung H. H., . and Chow K. S., . and Thompson C., . and Mollison E., . Identification and characterisation of endo-1 4--glucanase family members in Hevea brasiliensis. pp. 148-158. ISSN 1511-1768
Abstract
In order to study wood formation in Hevea brasiliensis endo-1 4--glucanase (EGase) was selected for molecular genetic analysis. EGase sequences were isolated from bark leaf and latex transcriptomes (clone RRIM 928). Altogether 22 sequences were obtained from the bark transcriptome while 14 and 8 sequences were obtained from leaf and latex transcriptomes respectively. Based on multiple sequence alignment only twelve sequences encoded full protein open reading frames while the others contained partial proteins. Translation of twelve EGase sequences (designated HbEg1-12) produced peptide sequences ranging from 494-622 amino acids. Based on protein motif conservation and phylogenetic analysis these EGases were separated into three classes A B and C. Expression of EGase was found to be generally low in latex bark and leaves.
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Abstract
In order to study wood formation in Hevea brasiliensis endo-1 4--glucanase (EGase) was selected for molecular genetic analysis. EGase sequences were isolated from bark leaf and latex transcriptomes (clone RRIM 928). Altogether 22 sequences were obtained from the bark transcriptome while 14 and 8 sequences were obtained from leaf and latex transcriptomes respectively. Based on multiple sequence alignment only twelve sequences encoded full protein open reading frames while the others contained partial proteins. Translation of twelve EGase sequences (designated HbEg1-12) produced peptide sequences ranging from 494-622 amino acids. Based on protein motif conservation and phylogenetic analysis these EGases were separated into three classes A B and C. Expression of EGase was found to be generally low in latex bark and leaves.
Additional Metadata
| Item Type: | Article |
|---|---|
| AGROVOC Term: | Identification |
| AGROVOC Term: | Beta glucanase |
| AGROVOC Term: | Hevea brasiliensis |
| AGROVOC Term: | Rubber |
| AGROVOC Term: | Wood formation |
| AGROVOC Term: | Molecular genetics |
| AGROVOC Term: | Clones |
| AGROVOC Term: | Amino acids |
| AGROVOC Term: | Latex |
| AGROVOC Term: | Leaves |
| Geographical Term: | Malaysia |
| Depositing User: | Ms. Suzila Mohamad Kasim |
| Last Modified: | 28 Apr 2025 00:41 |
| URI: | http://webagris.upm.edu.my/id/eprint/22941 |
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