Citation
Nurul Aishah Y., . and Sanimah S., . and Qistina O., . and Maheswary V., . and Khairun H. N., . and Vasanthi S., . Transcriptome analysis of floral scent biosynthesis during Michelia alba (cempaka putih) flower development. pp. 37-54. ISSN 1394-9829
Abstract
Despite a long history of cultivation of Michelia alba (cempaka putih) and the commercial importance of its essential oils no information is currently available on potential changes in the expression of genes involved in the synthesis ofindividual volatile organic compounds (VOCs) during flower development. Here we describe the combined use of Digital Gene Expression (DGE) and chemical analysis (from a previous study) to discover genes for the VOCs biosynthesis pathways and related genes. From the DGE sequencing data a total of 50 genes (including isoforms) were identified to be involved directly with fragrance biosynthesis from the pooled early stages (S1 “ S6) and pooled late stages (S7 “ S11) of flower development. Our transcriptome data revealed that the bulk of floral scent genes (29) in this species belonged to the isoprenoids biosynthesis pathway of which 15 transcripts belonged to the monoterpeoids biosynthesis pathway seven belonged to the sesquiterpenoids biosythesis pathway while the remaining seven belonged to the terpenoids backbone biosynthesis pathway. Fifteen genes were also identified to be involved in the biosynthesis of aromatic compounds in the phenylpropanoids/benzenoids biosynthesis pathway while six were found to belong to the fatty acid derivatives biosythesis pathway. Four other other genes were also identified to be related to floral scent biosynthesis in other metabolic pathways.
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Official URL: http://jtafs.mardi.gov.my/jtafs/47-2/michelia.pdf
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Abstract
Despite a long history of cultivation of Michelia alba (cempaka putih) and the commercial importance of its essential oils no information is currently available on potential changes in the expression of genes involved in the synthesis ofindividual volatile organic compounds (VOCs) during flower development. Here we describe the combined use of Digital Gene Expression (DGE) and chemical analysis (from a previous study) to discover genes for the VOCs biosynthesis pathways and related genes. From the DGE sequencing data a total of 50 genes (including isoforms) were identified to be involved directly with fragrance biosynthesis from the pooled early stages (S1 “ S6) and pooled late stages (S7 “ S11) of flower development. Our transcriptome data revealed that the bulk of floral scent genes (29) in this species belonged to the isoprenoids biosynthesis pathway of which 15 transcripts belonged to the monoterpeoids biosynthesis pathway seven belonged to the sesquiterpenoids biosythesis pathway while the remaining seven belonged to the terpenoids backbone biosynthesis pathway. Fifteen genes were also identified to be involved in the biosynthesis of aromatic compounds in the phenylpropanoids/benzenoids biosynthesis pathway while six were found to belong to the fatty acid derivatives biosythesis pathway. Four other other genes were also identified to be related to floral scent biosynthesis in other metabolic pathways.
Additional Metadata
Item Type: | Article |
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AGROVOC Term: | Michelia |
AGROVOC Term: | Magnoliaceae |
AGROVOC Term: | Floral initiation |
AGROVOC Term: | Scent |
AGROVOC Term: | Biosynthesis |
AGROVOC Term: | Flowering |
AGROVOC Term: | analysis |
AGROVOC Term: | Sampling |
AGROVOC Term: | Monoterpenoids |
AGROVOC Term: | Lipoxygenase |
Depositing User: | Mr. AFANDI ABDUL MALEK |
Last Modified: | 24 Apr 2025 00:54 |
URI: | http://webagris.upm.edu.my/id/eprint/9088 |
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