Transcriptome analysis of floral scent biosynthesis during Michelia alba (cempaka putih) flower development

Nurul Aishah Y., . and Sanimah S., . and Qistina O., . and Maheswary V., . and Khairun H. N., . and Vasanthi S., . Transcriptome analysis of floral scent biosynthesis during Michelia alba (cempaka putih) flower development. pp. 37-54. ISSN 1394-9829

Despite a long history of cultivation of Michelia alba (cempaka putih) and the commercial importance of its essential oils no information is currently available on potential changes in the expression of genes involved in the synthesis ofindividual volatile organic compounds (VOCs) during flower development. Here we describe the combined use of Digital Gene Expression (DGE) and chemical analysis (from a previous study) to discover genes for the VOCs biosynthesis pathways and related genes. From the DGE sequencing data a total of 50 genes (including isoforms) were identified to be involved directly with fragrance biosynthesis from the pooled early stages (S1 “ S6) and pooled late stages (S7 “ S11) of flower development. Our transcriptome data revealed that the bulk of floral scent genes (29) in this species belonged to the isoprenoids biosynthesis pathway of which 15 transcripts belonged to the monoterpeoids biosynthesis pathway seven belonged to the sesquiterpenoids biosythesis pathway while the remaining seven belonged to the terpenoids backbone biosynthesis pathway. Fifteen genes were also identified to be involved in the biosynthesis of aromatic compounds in the phenylpropanoids/benzenoids biosynthesis pathway while six were found to belong to the fatty acid derivatives biosythesis pathway. Four other other genes were also identified to be related to floral scent biosynthesis in other metabolic pathways.

Full text available from: Official URL: http://jtafs.mardi.gov.my/jtafs/47-2/michelia.pdf

Abstract

Despite a long history of cultivation of Michelia alba (cempaka putih) and the commercial importance of its essential oils no information is currently available on potential changes in the expression of genes involved in the synthesis ofindividual volatile organic compounds (VOCs) during flower development. Here we describe the combined use of Digital Gene Expression (DGE) and chemical analysis (from a previous study) to discover genes for the VOCs biosynthesis pathways and related genes. From the DGE sequencing data a total of 50 genes (including isoforms) were identified to be involved directly with fragrance biosynthesis from the pooled early stages (S1 “ S6) and pooled late stages (S7 “ S11) of flower development. Our transcriptome data revealed that the bulk of floral scent genes (29) in this species belonged to the isoprenoids biosynthesis pathway of which 15 transcripts belonged to the monoterpeoids biosynthesis pathway seven belonged to the sesquiterpenoids biosythesis pathway while the remaining seven belonged to the terpenoids backbone biosynthesis pathway. Fifteen genes were also identified to be involved in the biosynthesis of aromatic compounds in the phenylpropanoids/benzenoids biosynthesis pathway while six were found to belong to the fatty acid derivatives biosythesis pathway. Four other other genes were also identified to be related to floral scent biosynthesis in other metabolic pathways.

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Item Type:	Article
AGROVOC Term:	Michelia
AGROVOC Term:	Magnoliaceae
AGROVOC Term:	Floral initiation
AGROVOC Term:	Scent
AGROVOC Term:	Biosynthesis
AGROVOC Term:	Flowering
AGROVOC Term:	analysis
AGROVOC Term:	Sampling
AGROVOC Term:	Monoterpenoids
AGROVOC Term:	Lipoxygenase
Depositing User:	Mr. AFANDI ABDUL MALEK
Last Modified:	24 Apr 2025 00:54
URI:	http://webagris.upm.edu.my/id/eprint/9088

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