Citation
Lu H., . and Li J., . and Wang X. S., . and Wu Y. F., . and Deng R. X., . Optimisation of parameters antioxidant activity and component analysis of flavonoids from Ecliptae Herba. pp. 745-753. ISSN 2231-7546
Abstract
Ultrasonic probe extraction of total flavonoids from Ecliptae Herba (EH) was investigated using response surface methodology. The parameters based on Plackett-Burman design were optimised with the Box-Behnken design to improve the extraction yield. The optimal extraction conditions were as follows: extraction solvent of water ultrasound frequency of 40 kHz number of extractions of 3 extraction temperature of 60C and solvent-material ratio of 30:1 mL/g. Under these conditions the experimental extraction yield was 42.62 1.38 mg/g which matched well with the predicted value of 43.74 mg/g. Conventional reflux extraction was compared to ultrasonic probe extraction. In addition scanning electron microscopy (SEM) imaging of EH after extraction was carried out. The antioxidant capacity of total flavonoids was evaluated using DPPH ABTS superoxide anion and reducing power assays which suggested that the flavonoid extract from EH can be used as a potential antioxidant additive in the food and pharmaceutical industries. Furthermore qualitative and quantitative analyses of the flavonoid extract were studied.
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Abstract
Ultrasonic probe extraction of total flavonoids from Ecliptae Herba (EH) was investigated using response surface methodology. The parameters based on Plackett-Burman design were optimised with the Box-Behnken design to improve the extraction yield. The optimal extraction conditions were as follows: extraction solvent of water ultrasound frequency of 40 kHz number of extractions of 3 extraction temperature of 60C and solvent-material ratio of 30:1 mL/g. Under these conditions the experimental extraction yield was 42.62 1.38 mg/g which matched well with the predicted value of 43.74 mg/g. Conventional reflux extraction was compared to ultrasonic probe extraction. In addition scanning electron microscopy (SEM) imaging of EH after extraction was carried out. The antioxidant capacity of total flavonoids was evaluated using DPPH ABTS superoxide anion and reducing power assays which suggested that the flavonoid extract from EH can be used as a potential antioxidant additive in the food and pharmaceutical industries. Furthermore qualitative and quantitative analyses of the flavonoid extract were studied.
Additional Metadata
Item Type: | Article |
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AGROVOC Term: | Asteraceae |
AGROVOC Term: | Medicinal plants |
AGROVOC Term: | Flavonoids |
AGROVOC Term: | Extraction |
AGROVOC Term: | Centrifuges |
AGROVOC Term: | Scanning microscopy |
AGROVOC Term: | Methodology |
AGROVOC Term: | Ultrasound |
AGROVOC Term: | Plant extracts |
AGROVOC Term: | Antioxidants |
Depositing User: | Mr. AFANDI ABDUL MALEK |
Last Modified: | 24 Apr 2025 00:54 |
URI: | http://webagris.upm.edu.my/id/eprint/9285 |
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