Optimisation of parameters antioxidant activity and component analysis of flavonoids from Ecliptae Herba


Citation

Lu H., . and Li J., . and Wang X. S., . and Wu Y. F., . and Deng R. X., . Optimisation of parameters antioxidant activity and component analysis of flavonoids from Ecliptae Herba. pp. 745-753. ISSN 2231-7546

Abstract

Ultrasonic probe extraction of total flavonoids from Ecliptae Herba (EH) was investigated using response surface methodology. The parameters based on Plackett-Burman design were optimised with the Box-Behnken design to improve the extraction yield. The optimal extraction conditions were as follows: extraction solvent of water ultrasound frequency of 40 kHz number of extractions of 3 extraction temperature of 60C and solvent-material ratio of 30:1 mL/g. Under these conditions the experimental extraction yield was 42.62 1.38 mg/g which matched well with the predicted value of 43.74 mg/g. Conventional reflux extraction was compared to ultrasonic probe extraction. In addition scanning electron microscopy (SEM) imaging of EH after extraction was carried out. The antioxidant capacity of total flavonoids was evaluated using DPPH ABTS superoxide anion and reducing power assays which suggested that the flavonoid extract from EH can be used as a potential antioxidant additive in the food and pharmaceutical industries. Furthermore qualitative and quantitative analyses of the flavonoid extract were studied.


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Abstract

Ultrasonic probe extraction of total flavonoids from Ecliptae Herba (EH) was investigated using response surface methodology. The parameters based on Plackett-Burman design were optimised with the Box-Behnken design to improve the extraction yield. The optimal extraction conditions were as follows: extraction solvent of water ultrasound frequency of 40 kHz number of extractions of 3 extraction temperature of 60C and solvent-material ratio of 30:1 mL/g. Under these conditions the experimental extraction yield was 42.62 1.38 mg/g which matched well with the predicted value of 43.74 mg/g. Conventional reflux extraction was compared to ultrasonic probe extraction. In addition scanning electron microscopy (SEM) imaging of EH after extraction was carried out. The antioxidant capacity of total flavonoids was evaluated using DPPH ABTS superoxide anion and reducing power assays which suggested that the flavonoid extract from EH can be used as a potential antioxidant additive in the food and pharmaceutical industries. Furthermore qualitative and quantitative analyses of the flavonoid extract were studied.

Additional Metadata

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Item Type: Article
AGROVOC Term: Asteraceae
AGROVOC Term: Medicinal plants
AGROVOC Term: Flavonoids
AGROVOC Term: Extraction
AGROVOC Term: Centrifuges
AGROVOC Term: Scanning microscopy
AGROVOC Term: Methodology
AGROVOC Term: Ultrasound
AGROVOC Term: Plant extracts
AGROVOC Term: Antioxidants
Depositing User: Mr. AFANDI ABDUL MALEK
Last Modified: 24 Apr 2025 00:54
URI: http://webagris.upm.edu.my/id/eprint/9285

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