Hydroethanolic leaf extract of Parthenium hysterophorus attenuates blood glucose in alloxan induced diabetic mice.

Farhan M., . and Raza S. A., . and Ali S., . and Bashir S., . and Ahmad M., . and William J., . Hydroethanolic leaf extract of Parthenium hysterophorus attenuates blood glucose in alloxan induced diabetic mice. pp. 72-77. ISSN 2550-2166

The Parthenium hysterophorus (P. hysterophorus) is used to treat diabetes mellitus in local medicinal system of Pakistan but very limited scientifically proved information is available in this context. The objective of this work was to evaluate the antioxidant and antidiabetic activity of P. hysterophorus leaf extract. The extraction was made with freeze drying assisted ultrasonication using 40 60 and 80 ethanol as solvent. The total phenolic and flavonoid contents were calculated. Antioxidant activity was determined by 2 2-diphenyl-1-picrylhydrazyl (DPPH) assay and -glucosidase inhibitory assay was also performed. The in vivo hypoglycemic potential of leaf extract was determined in alloxan monohydrate induced diabetic mice. The 60 ethanolic extract exhibited comparatively higher phenolic and flavonoid contents with values of 105.442.55 mg GAE/g D.E and 41.502.25 mg RE/g D.E respectively. The 60 extract also possessed lowest IC‚‚ value of 87.55 g/mL and 98.22 g/mL for DPPH radical scavenging and -glucosidase inhibition respectively. The same extract substantially reduced the blood glucose level in alloxan induced diabetic mice and results were quite comparable with standard drug metformin. The extract dose of 450 mg/kg reduced the blood glucose level of diabetic mice from 268.05 mg/dL 137.88 mg/dL at the end of 28 days treatment. The findings confirmed the ethnopharmacological use of P. hysterophorus to treat and manage diabetes mellitus type 2. The experimental outcomes may be employed as pharmacological leads to treat diabetes mellitus and to develop functional foods with hypoglycemic attributes.

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Abstract

The Parthenium hysterophorus (P. hysterophorus) is used to treat diabetes mellitus in local medicinal system of Pakistan but very limited scientifically proved information is available in this context. The objective of this work was to evaluate the antioxidant and antidiabetic activity of P. hysterophorus leaf extract. The extraction was made with freeze drying assisted ultrasonication using 40 60 and 80 ethanol as solvent. The total phenolic and flavonoid contents were calculated. Antioxidant activity was determined by 2 2-diphenyl-1-picrylhydrazyl (DPPH) assay and -glucosidase inhibitory assay was also performed. The in vivo hypoglycemic potential of leaf extract was determined in alloxan monohydrate induced diabetic mice. The 60 ethanolic extract exhibited comparatively higher phenolic and flavonoid contents with values of 105.442.55 mg GAE/g D.E and 41.502.25 mg RE/g D.E respectively. The 60 extract also possessed lowest IC‚‚ value of 87.55 g/mL and 98.22 g/mL for DPPH radical scavenging and -glucosidase inhibition respectively. The same extract substantially reduced the blood glucose level in alloxan induced diabetic mice and results were quite comparable with standard drug metformin. The extract dose of 450 mg/kg reduced the blood glucose level of diabetic mice from 268.05 mg/dL 137.88 mg/dL at the end of 28 days treatment. The findings confirmed the ethnopharmacological use of P. hysterophorus to treat and manage diabetes mellitus type 2. The experimental outcomes may be employed as pharmacological leads to treat diabetes mellitus and to develop functional foods with hypoglycemic attributes.

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Item Type:	Article
AGROVOC Term:	Diabetes mellitus
AGROVOC Term:	Antioxidants
AGROVOC Term:	Extraction
AGROVOC Term:	Sampling
AGROVOC Term:	Experimental design
AGROVOC Term:	Freeze-drying
AGROVOC Term:	Functional foods
Depositing User:	Mr. AFANDI ABDUL MALEK
Last Modified:	24 Apr 2025 00:55
URI:	http://webagris.upm.edu.my/id/eprint/10166

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