Glycan characterisation and antioxidant activity of a novel N-linked glycoprotein from okra

Chen Y., . and Xu G., . and Li J., . and Yu H., . and Zhao W., . and Liao X., . and Yu Z., . Glycan characterisation and antioxidant activity of a novel N-linked glycoprotein from okra. pp. 1119-1130. ISSN 22317546

Glycoproteins are present in all living beings and have many biological functions. The characterisation of glycan structures of plant glycoproteins has become increasingly important in biotechnology and agricultural applications. In the present work the antioxidant activities of the okra glycoprotein were assessed. The glycan structures of the okra glycoprotein were analysed using lectin microarray combined with matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry. The okra glycoprotein showed relatively strong 2 2-diphenyl-1-picrylhydrazyl-scavenging ability and reducing power. In addition the glycan structures of the okra glycoprotein mainly contained N-acetylglucosamine mannose and galactose. Furthermore complex-type N-glycans were the major type of glycan structures from the okra glycoprotein. Most of the complex N-glycans of the okra glycoprotein had terminal GalNAc and Gal N-glycan structures; the glycoprotein showed a high level of fucosylated complex-type glycans. Therefore the okra glycoprotein is a promising antioxidant. Results of the present work might serve as a reference for a better understanding of the structural information and bioactivity of okra glycoprotein.

Full text available from: Official URL: http://www.ifrj.upm.edu.my/28%20(06)%202021/DONE%2...

Abstract

Glycoproteins are present in all living beings and have many biological functions. The characterisation of glycan structures of plant glycoproteins has become increasingly important in biotechnology and agricultural applications. In the present work the antioxidant activities of the okra glycoprotein were assessed. The glycan structures of the okra glycoprotein were analysed using lectin microarray combined with matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry. The okra glycoprotein showed relatively strong 2 2-diphenyl-1-picrylhydrazyl-scavenging ability and reducing power. In addition the glycan structures of the okra glycoprotein mainly contained N-acetylglucosamine mannose and galactose. Furthermore complex-type N-glycans were the major type of glycan structures from the okra glycoprotein. Most of the complex N-glycans of the okra glycoprotein had terminal GalNAc and Gal N-glycan structures; the glycoprotein showed a high level of fucosylated complex-type glycans. Therefore the okra glycoprotein is a promising antioxidant. Results of the present work might serve as a reference for a better understanding of the structural information and bioactivity of okra glycoprotein.

[error in script]

Item Type:	Article
AGROVOC Term:	Glycoproteins
AGROVOC Term:	Antioxidants
AGROVOC Term:	Biochemistry
AGROVOC Term:	Okras
AGROVOC Term:	analysis
AGROVOC Term:	Food industry
Depositing User:	Mr. AFANDI ABDUL MALEK
Last Modified:	24 Apr 2025 00:55
URI:	http://webagris.upm.edu.my/id/eprint/10300

Actions (login required)

View Item