Determination of antiradical activity and phenolic and flavonoid contents of extracts and fractions of jackfruit (Artocarpus heterophyllus Lamk) seeds

Zubaydah W. O. S., . and Sahumena M. H., . and Fatimah W. O. N., . and Sabarudin., . and Arba M., . and Yamin., . Determination of antiradical activity and phenolic and flavonoid contents of extracts and fractions of jackfruit (Artocarpus heterophyllus Lamk) seeds. pp. 36-43. ISSN 2550-2166

Jackfruit (Artocarpus heterophyllus Lamk) is a plant from the Moraceae family that is widespread in Indonesia. Empirically jackfruit can be used to cure hypertension diabetes cancer asthma dermatosis coughs wounds acne and diarrhea. The bioactive compounds in jackfruit include phenolics and flavonoids which function as natural antioxidants. This research investigated the antiradical activity of jackfruit seed extracts and fractions using DPPH (2-2-diphenyl-1-picrylhydrazyl) and examined the total phenolic and flavonoid contents which may be developed as functional medicines and foods. The jackfruit seed powder was extracted using the maceration method. Radical scavenging activities were measured using the 2 2-diphenyl-1-picrylhydrazyl (DPPH) method. Total phenolic contents were determined using the Folin-Ciocalteu method. Meanwhile the contents of flavonoids were determined using the aluminum chloride complex colorimetric method. The IC‚‚ value of ethyl acetate fraction methanol extract n -hexane fraction and water fraction was 5.4350.064 g/mL 5.6390.302 g/mL 7.2010.475 g/mL and 9.1340.2911 g/mL respectively. The phenolic and flavonoid contents of ethyl acetate fraction methanol extract n-hexane fraction and water fraction were 49.5971.589 47.9491.966 41.2144.354 and 35.5040.913 g GAE/100 g of sample respectively for phenolic and 70.1990.458 65.2280.615 59.9070.719 and 54.2340.351 g of quercetin equivalent/100 g of sample respectively of flavonoids with a correlation value (R) to antiradical power of 0.4582 for phenolic and 0.5281 for flavonoids. The ethyl acetate fraction of jackfruit seeds can be further developed as an anti -radicals and functional food.

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Abstract

Jackfruit (Artocarpus heterophyllus Lamk) is a plant from the Moraceae family that is widespread in Indonesia. Empirically jackfruit can be used to cure hypertension diabetes cancer asthma dermatosis coughs wounds acne and diarrhea. The bioactive compounds in jackfruit include phenolics and flavonoids which function as natural antioxidants. This research investigated the antiradical activity of jackfruit seed extracts and fractions using DPPH (2-2-diphenyl-1-picrylhydrazyl) and examined the total phenolic and flavonoid contents which may be developed as functional medicines and foods. The jackfruit seed powder was extracted using the maceration method. Radical scavenging activities were measured using the 2 2-diphenyl-1-picrylhydrazyl (DPPH) method. Total phenolic contents were determined using the Folin-Ciocalteu method. Meanwhile the contents of flavonoids were determined using the aluminum chloride complex colorimetric method. The IC‚‚ value of ethyl acetate fraction methanol extract n -hexane fraction and water fraction was 5.4350.064 g/mL 5.6390.302 g/mL 7.2010.475 g/mL and 9.1340.2911 g/mL respectively. The phenolic and flavonoid contents of ethyl acetate fraction methanol extract n-hexane fraction and water fraction were 49.5971.589 47.9491.966 41.2144.354 and 35.5040.913 g GAE/100 g of sample respectively for phenolic and 70.1990.458 65.2280.615 59.9070.719 and 54.2340.351 g of quercetin equivalent/100 g of sample respectively of flavonoids with a correlation value (R) to antiradical power of 0.4582 for phenolic and 0.5281 for flavonoids. The ethyl acetate fraction of jackfruit seeds can be further developed as an anti -radicals and functional food.

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Item Type:	Article
AGROVOC Term:	Jack fruit
AGROVOC Term:	Artocarpus heterophyllus
AGROVOC Term:	Moraceae
AGROVOC Term:	Diphenyl
AGROVOC Term:	Phenolic content
AGROVOC Term:	Flavonoids
AGROVOC Term:	Macerating
AGROVOC Term:	Seed extraction
AGROVOC Term:	Functional foods
AGROVOC Term:	Natural antioxidants
Depositing User:	Mr. AFANDI ABDUL MALEK
Last Modified:	24 Apr 2025 00:55
URI:	http://webagris.upm.edu.my/id/eprint/10454

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