Phytochemical analysis total phenolic total flavonoid contents and ferric reducing power of extracts from roots and leaves of Searsia burchellii


Citation

Pillai M. K., . and Mpopo M. S., . and Mekbib. S. B., . Phytochemical analysis total phenolic total flavonoid contents and ferric reducing power of extracts from roots and leaves of Searsia burchellii. pp. 241-248. ISSN 2550-2166

Abstract

Searsia burchellii has therapeutic applications in traditional medicine. The aim of the present study was to analyse the phytochemical profiles total phenolic contents (TPC) total flavonoid contents (TFC) and ferric reducing power of various extracts from roots and leaves of S. burchellii collected from the kingdom of Lesotho. A combination of maceration hot solvent extraction and solvent-solvent partition techniques were used to obtain methanolic extracts fractions such as hexane chloroform ethyl acetate and methanol/water from methanolic extracts and water extracts separately from the roots and leaves of S. burchellii. The presence of alkaloids steroids terpenoids phenolics tannins flavonoids coumarins saponins glycosides carbohydrates proteins and phlobatannins were identified from these extracts/fractions. The TPC of these extracts/fractions was found to be in the range of 3.220.37 to 247.464.33 mg TAE/g DW respectively. The TFC of these extracts/fractions was found to be in the range of 34.704.31 to 278.202.01 mg QE/g DW respectively. Additionally these extracts/fractions showed a moderate to strong ferric reducing power compared to ascorbic acid. The IC50 values were found to be in the range of 5.35-143.12 and 65.64-348.28 g/mL for extracts/fractions from the roots and leaves respectively. Ascorbic acid showed an IC‚‚ value of 20.93 g/mL. Therefore the extracts/fractions from S. burchellii have the potential to replace the existing synthetic antioxidants.


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Abstract

Searsia burchellii has therapeutic applications in traditional medicine. The aim of the present study was to analyse the phytochemical profiles total phenolic contents (TPC) total flavonoid contents (TFC) and ferric reducing power of various extracts from roots and leaves of S. burchellii collected from the kingdom of Lesotho. A combination of maceration hot solvent extraction and solvent-solvent partition techniques were used to obtain methanolic extracts fractions such as hexane chloroform ethyl acetate and methanol/water from methanolic extracts and water extracts separately from the roots and leaves of S. burchellii. The presence of alkaloids steroids terpenoids phenolics tannins flavonoids coumarins saponins glycosides carbohydrates proteins and phlobatannins were identified from these extracts/fractions. The TPC of these extracts/fractions was found to be in the range of 3.220.37 to 247.464.33 mg TAE/g DW respectively. The TFC of these extracts/fractions was found to be in the range of 34.704.31 to 278.202.01 mg QE/g DW respectively. Additionally these extracts/fractions showed a moderate to strong ferric reducing power compared to ascorbic acid. The IC50 values were found to be in the range of 5.35-143.12 and 65.64-348.28 g/mL for extracts/fractions from the roots and leaves respectively. Ascorbic acid showed an IC‚‚ value of 20.93 g/mL. Therefore the extracts/fractions from S. burchellii have the potential to replace the existing synthetic antioxidants.

Additional Metadata

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Item Type: Article
AGROVOC Term: Anacardiaceae
AGROVOC Term: Rhus
AGROVOC Term: Leaves
AGROVOC Term: Roots
AGROVOC Term: Plant extracts
AGROVOC Term: Extraction
AGROVOC Term: Phytochemistry
AGROVOC Term: Flavonoids
AGROVOC Term: Ascorbic acid
AGROVOC Term: Phenolic content
Depositing User: Mr. AFANDI ABDUL MALEK
Last Modified: 24 Apr 2025 00:55
URI: http://webagris.upm.edu.my/id/eprint/10477

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