All-in-one comprehensive extraction of metabolites proteins and ribonucleic acid for the rapid analysis of oil palm systems biology

Nurazah Zain, . and Abrizah Othman, . and Nurul Liyana Rozali, . and Umi Salamah Ramli, . and Syahanim Shahwan, . All-in-one comprehensive extraction of metabolites proteins and ribonucleic acid for the rapid analysis of oil palm systems biology. pp. 234-247. ISSN 2811-4701

Oil palm (Elaeis guineensis Jacq.) systems biology offers a comprehensive view of the plant system by employing a holistic multi-omics approach encompassing the molecular data at various hierarchical levels. Sample limitation and the importance of integrating all molecular data with minimal variation led to the development of sequential extraction of biomolecules fractions from a single undivided biological sample. Oil palm root was subjected to solvent extraction with methanol-chloroform-water to recover metabolites of diverse polarity. The resultant pellet was subjected to buffer and solvent partitioning to obtain RNA and proteins. RNA extracted from the oil palm root showed a recovery of 180.25 ng mg“ with an A260:A280 ratio ranging between 1.9-2.0 and RNA integrity number (RIN) value of 6.7. Co-extracted proteins resulted in a recovery of 29.28 g per 100 mg fresh weight (FW) tissue and revealed a total of 1852 identified proteins in the oil palm root. Polar metabolites revealed approximately 40 metabolite peaks and non-polar metabolites with two major fatty acid groups i.e. saturated and unsaturated fatty acids at 55.4 and 38.6 respectively. This protocol demonstrated an advancement of extraction protocols for oil palm root biomolecules which will consecutively expedite the establishment of various multi-omics platforms.

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Abstract

Oil palm (Elaeis guineensis Jacq.) systems biology offers a comprehensive view of the plant system by employing a holistic multi-omics approach encompassing the molecular data at various hierarchical levels. Sample limitation and the importance of integrating all molecular data with minimal variation led to the development of sequential extraction of biomolecules fractions from a single undivided biological sample. Oil palm root was subjected to solvent extraction with methanol-chloroform-water to recover metabolites of diverse polarity. The resultant pellet was subjected to buffer and solvent partitioning to obtain RNA and proteins. RNA extracted from the oil palm root showed a recovery of 180.25 ng mg“ with an A260:A280 ratio ranging between 1.9-2.0 and RNA integrity number (RIN) value of 6.7. Co-extracted proteins resulted in a recovery of 29.28 g per 100 mg fresh weight (FW) tissue and revealed a total of 1852 identified proteins in the oil palm root. Polar metabolites revealed approximately 40 metabolite peaks and non-polar metabolites with two major fatty acid groups i.e. saturated and unsaturated fatty acids at 55.4 and 38.6 respectively. This protocol demonstrated an advancement of extraction protocols for oil palm root biomolecules which will consecutively expedite the establishment of various multi-omics platforms.

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Item Type:	Article
AGROVOC Term:	Oil palm
AGROVOC Term:	Elaeis guineensis
AGROVOC Term:	Roots
AGROVOC Term:	Extraction
AGROVOC Term:	Proteins
AGROVOC Term:	Mesocarp
AGROVOC Term:	Ribonucleic acid
AGROVOC Term:	Agricultural systems
Depositing User:	Mr. AFANDI ABDUL MALEK
Last Modified:	24 Apr 2025 00:55
URI:	http://webagris.upm.edu.my/id/eprint/10674

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