Microbial protein extraction from palm oil mill effluent

Nor Faizah J., . and Noorshamsiana A. W., . and Wan Hasamudin W. H., . and Nahrul Hayawin Z., . and Ropandi M., . and Nur Eliyanti A. O., . and Astimar A. A., . and Rohaya M. H., . Microbial protein extraction from palm oil mill effluent. pp. 311-322. ISSN 2811-4701

Palm oil mill effluent (POME) is abundantly produced during palm oil milling process and is treated for pollutant reduction without any revenue or profit. The main objective of this research was to evaluate POME as a substrate for microbial protein production and analyse the removal rate of pollutant. This study used hydrolysed POME containing fermentable sugars as carbon source for the cultivation of microbes. Raw POME was initially pre-treated with Celluclast enzyme using the following conditions: Concentration (1.5 to 5.0 v/v); incubation temperature (45„-55„) rotation speed (100-200 rpm) and pH (4.0-5.5). After pre-treatment the hydrolysate contained 41.63 g L“ reducing sugars with 56 000 mg L“ chemical oxygen demand (COD). Bakers Yeast (Saccharomyces cerevisiae) was then cultivated onto the raw or hydrolysed POME at 30„ for seven days. The amount of yeast biomass produced was 28.92 g L“ with 24.79 protein content. Adding to this the COD value was reduced by 79.12. The yeast fermentation in hydrolysed POME recorded the highest increase in biomass and protein contents of 3.44 and 7.74 folds respectively. The findings revealed that POME is a promising raw material for microbial biomass protein production and simultaneously remove the pollutant from POME.

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Abstract

Palm oil mill effluent (POME) is abundantly produced during palm oil milling process and is treated for pollutant reduction without any revenue or profit. The main objective of this research was to evaluate POME as a substrate for microbial protein production and analyse the removal rate of pollutant. This study used hydrolysed POME containing fermentable sugars as carbon source for the cultivation of microbes. Raw POME was initially pre-treated with Celluclast enzyme using the following conditions: Concentration (1.5 to 5.0 v/v); incubation temperature (45„-55„) rotation speed (100-200 rpm) and pH (4.0-5.5). After pre-treatment the hydrolysate contained 41.63 g L“ reducing sugars with 56 000 mg L“ chemical oxygen demand (COD). Bakers Yeast (Saccharomyces cerevisiae) was then cultivated onto the raw or hydrolysed POME at 30„ for seven days. The amount of yeast biomass produced was 28.92 g L“ with 24.79 protein content. Adding to this the COD value was reduced by 79.12. The yeast fermentation in hydrolysed POME recorded the highest increase in biomass and protein contents of 3.44 and 7.74 folds respectively. The findings revealed that POME is a promising raw material for microbial biomass protein production and simultaneously remove the pollutant from POME.

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Item Type:	Article
AGROVOC Term:	Palm oils
AGROVOC Term:	Milling byproducts
AGROVOC Term:	Extraction
AGROVOC Term:	Microbial proteins (product)
AGROVOC Term:	Biomass
AGROVOC Term:	Fermentation
AGROVOC Term:	Protein content
AGROVOC Term:	Animal feed crops
AGROVOC Term:	Biological treatment of pollutants
Depositing User:	Mr. AFANDI ABDUL MALEK
Last Modified:	24 Apr 2025 00:55
URI:	http://webagris.upm.edu.my/id/eprint/10680

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