Proximate composition and changes in muscle proteins of dried salted abo (Otolithes ruber)

Canon K. L., . and Bigueja M. C., . and Avila T. N., . and Bradecina R. G., . and Mendoza Jr. A. B., . and Kubota S., . Proximate composition and changes in muscle proteins of dried salted abo (Otolithes ruber). pp. 178-187. ISSN 2550-2166

Dried salted abo is a popular fishery product in San Miguel Bay (SMB) Philippines. However the development of this food product has not been fully explored. In this study the proximate composition and muscle protein profile of dried salted abo was investigated after confirming the molecular identification of abo found in SMB. Sequence analysis of 16S ribosomal RNA and cytochrome c oxidase subunit I genes suggested that abo in SMB could be Otolithes ruber. Abo fillets were brined in 4 0.08 and 12 salt concentrations (w/w) and sun-dried for 6 and 12 hrs. In both 6 hrs and 12 hrs drying time moisture content decreased in samples brined with 12 salt suggesting that the salt concentration could be lowered between 8“12 with 6 hrs drying time. Additionally the proteolytic profiling by SDS-PAGE analysis detected 17 bands in the myofibrillar protein fraction. Subsequent mass spectrometry identified nine proteins of which the myosin heavy chain actin and keratin were degraded while the other proteins remained stable. Moreover at 12 salt concentration changes in staining intensities in several proteins were observed at both 6 hrs and 12 hrs drying time. These findings revealed that the applied salting and drying conditions led to the degradation of myosin heavy chain and polymerization of actin in addition to an overall alteration of protein profile which could affect the texture of dried abo product. Overall this study demonstrated molecular evidence for species identification of abo for the first time. The information on proximate composition and changes in the protein profile of dried salted abo obtained from this study could help the local dried fish industry develop high-quality dried abo products with low salt content.

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Abstract

Dried salted abo is a popular fishery product in San Miguel Bay (SMB) Philippines. However the development of this food product has not been fully explored. In this study the proximate composition and muscle protein profile of dried salted abo was investigated after confirming the molecular identification of abo found in SMB. Sequence analysis of 16S ribosomal RNA and cytochrome c oxidase subunit I genes suggested that abo in SMB could be Otolithes ruber. Abo fillets were brined in 4 0.08 and 12 salt concentrations (w/w) and sun-dried for 6 and 12 hrs. In both 6 hrs and 12 hrs drying time moisture content decreased in samples brined with 12 salt suggesting that the salt concentration could be lowered between 8“12 with 6 hrs drying time. Additionally the proteolytic profiling by SDS-PAGE analysis detected 17 bands in the myofibrillar protein fraction. Subsequent mass spectrometry identified nine proteins of which the myosin heavy chain actin and keratin were degraded while the other proteins remained stable. Moreover at 12 salt concentration changes in staining intensities in several proteins were observed at both 6 hrs and 12 hrs drying time. These findings revealed that the applied salting and drying conditions led to the degradation of myosin heavy chain and polymerization of actin in addition to an overall alteration of protein profile which could affect the texture of dried abo product. Overall this study demonstrated molecular evidence for species identification of abo for the first time. The information on proximate composition and changes in the protein profile of dried salted abo obtained from this study could help the local dried fish industry develop high-quality dried abo products with low salt content.

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Item Type:	Article
AGROVOC Term:	Otolithes ruber
AGROVOC Term:	Fish
AGROVOC Term:	Food preservation
AGROVOC Term:	Seafoods
AGROVOC Term:	Food quality
AGROVOC Term:	Mass spectrometry
AGROVOC Term:	Surveys
AGROVOC Term:	Sampling
AGROVOC Term:	Proximate analysis
AGROVOC Term:	Statistical analysis
Depositing User:	Mr. AFANDI ABDUL MALEK
Last Modified:	24 Apr 2025 00:55
URI:	http://webagris.upm.edu.my/id/eprint/10778

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