Cellular location of polyphenol oxidase in Metroxylon sagu


Citation

Galila H.O., . and Nazamid S., . and Shamsul Bahri A.R., . and Chan J.L., . and Jamilah B., . and Jinap S., . (2002) Cellular location of polyphenol oxidase in Metroxylon sagu. [Proceedings Paper]

Abstract

The location of polyphenol oxidase PPO in cells of sago pith tissues was examined histochemically and viwed under light microscope. In pith tissues collected from 3-4 years sago log a large number of vascular bundles and vacuoles occupied most of the cells. While in young sago pith 2-3 months the vacuoles occupied far less of the cytoplasm and dense pigmentations can be seen in some of the cytoplasmatic organelles. Using catechol for histochemical staining of PPO dense deposition of polyphenol oxidase reaction product is readily visualized within the xylem cell walls and the parenchyma cell walls. These results reveal that sago ptih browning is due to highly localized oxidative activity mediated by polyphenol oxidase.


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Abstract

The location of polyphenol oxidase PPO in cells of sago pith tissues was examined histochemically and viwed under light microscope. In pith tissues collected from 3-4 years sago log a large number of vascular bundles and vacuoles occupied most of the cells. While in young sago pith 2-3 months the vacuoles occupied far less of the cytoplasm and dense pigmentations can be seen in some of the cytoplasmatic organelles. Using catechol for histochemical staining of PPO dense deposition of polyphenol oxidase reaction product is readily visualized within the xylem cell walls and the parenchyma cell walls. These results reveal that sago ptih browning is due to highly localized oxidative activity mediated by polyphenol oxidase.

Additional Metadata

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Item Type: Proceedings Paper
Additional Information: 6 ill. 11 ref.
AGROVOC Term: CATECHOL OXIDASE
AGROVOC Term: METROXYLON
AGROVOC Term: MALAYSIA
Geographical Term: MALAYSIA
Depositing User: Ms. Suzila Mohamad Kasim
Last Modified: 24 Apr 2025 05:13
URI: http://webagris.upm.edu.my/id/eprint/11012

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