Evaluation of antioxidant capacity in three citrus species

Johari S., . and Nur Yuhasliza A R., . and Anisah J., . and Dang Lelamurni A R., . and Mohd Shaib J., . (2009) Evaluation of antioxidant capacity in three citrus species. [Proceedings Paper]

Studies have shown that increased consumption of fresh fruits and vegetables is associated with a lower incidence of disease and their protective mechanisms are thought to be attributed to the presence of natural antioxidants which help scavenge free radicals and reactive oxidants in the body. This study investigated the antioxidant capacity of three citrus fruit species namely Citrus microcarpa limau kasturi Citrus aurantifolia limau nipis and Cilrus hystrix limau purut. The antioxidant capacity of water and ethanolic extracts prepared from these species was measured by 1 1-diphenyl-2picrylhydrazyl radical-scavenging assay DPPH. In the ethanol extract the following hierarchy of antioxidant capacity was found: limau purut limau kasturi limau nipis with the value ranging from 0.39 0.02 to 0.28 0.02 mg Trolox/gfm. Whereas in the water extract limau purut still had the highest antioxidant activity but followed by limau nipis and limau kasturi with the value ranging from 0.63 0.01 to 0.16 0.03 mg Trolox/gfm. The antioxidant activity data in both extracts and species were also consistent with the total polyphenol content determined using the Folin-Ciocalteu method. In conclusion these three species possess valuable antioxidant properties and have high potential for the use of nutraceutical and functional food industries.

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Abstract

Studies have shown that increased consumption of fresh fruits and vegetables is associated with a lower incidence of disease and their protective mechanisms are thought to be attributed to the presence of natural antioxidants which help scavenge free radicals and reactive oxidants in the body. This study investigated the antioxidant capacity of three citrus fruit species namely Citrus microcarpa limau kasturi Citrus aurantifolia limau nipis and Cilrus hystrix limau purut. The antioxidant capacity of water and ethanolic extracts prepared from these species was measured by 1 1-diphenyl-2picrylhydrazyl radical-scavenging assay DPPH. In the ethanol extract the following hierarchy of antioxidant capacity was found: limau purut limau kasturi limau nipis with the value ranging from 0.39 0.02 to 0.28 0.02 mg Trolox/gfm. Whereas in the water extract limau purut still had the highest antioxidant activity but followed by limau nipis and limau kasturi with the value ranging from 0.63 0.01 to 0.16 0.03 mg Trolox/gfm. The antioxidant activity data in both extracts and species were also consistent with the total polyphenol content determined using the Folin-Ciocalteu method. In conclusion these three species possess valuable antioxidant properties and have high potential for the use of nutraceutical and functional food industries.

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Item Type:	Proceedings Paper
Additional Information:	Available at Perpustakaan Sultan Abdul Samad Universiti Putra Malaysia 43400 UPM Serdang Selangor Malaysia. mal QK99 M3S471 2008 Call Number
AGROVOC Term:	ANTIOXIDANTS
AGROVOC Term:	CITRUS
AGROVOC Term:	CITRUS AURANTIIFOLIA
AGROVOC Term:	EXTRACTS
AGROVOC Term:	MALAYSIA
Geographical Term:	MALAYSIA
Depositing User:	Ms. Suzila Mohamad Kasim
Last Modified:	24 Apr 2025 05:13
URI:	http://webagris.upm.edu.my/id/eprint/11224

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